Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis

Laura L. Carter, Thomas E. Redelmeier, Leslie A. Woollenweber, Sandra L. Schmid

Research output: Contribution to journalArticlepeer-review

159 Scopus citations


We have examined the effects of various agonists and antagonists of GTP-binding proteins on receptor-mediated endocytosis in vitro. Stage-specific assays which distinguish coated pit assembly, invagination, and coat vesicle budding have been used to demonstrate requirements for GTP-binding protein(s) in each of these events. Coated pit invagination and coated vesicle budding are both stimulated by addition of GTP and inhibited by GDPβS. Although coated pit invagination is resistant to GTP-γS, AlF4-, and mastoparan, late events involved in coated vesicle budding are inhibited by these antagonists of G protein function. Earlier events involved in coated pit assembly are also inhibited by GTPγS, AlF4-, and mastoparan. These results demonstrate that multiple GTP-binding proteins, including heterotrimeric G proteins, participate at discrete stages in receptor-mediated endocytosis via clathrin-coated pits.

Original languageEnglish (US)
Pages (from-to)37-45
Number of pages9
JournalJournal of Cell Biology
Issue number1
StatePublished - Jan 1993

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis'. Together they form a unique fingerprint.

Cite this