Multiomic Analysis of the UV-Induced DNA Damage Response

Stefan Boeing; Laura Williamson; Vesela Encheva; Ilaria Gori; Rebecca E. Saunders; Rachael Instrell; Ozan Aygün; Marta Rodriguez-Martinez; Juston C. Weems; Gavin P. Kelly; Joan W. Conaway; Ronald C. Conaway; Aengus Stewart; Michael Howell; Ambrosius P. Snijders; Jesper Q. Svejstrup

doi:10.1016/j.celrep.2016.04.047

Multiomic Analysis of the UV-Induced DNA Damage Response

Stefan Boeing, Laura Williamson, Vesela Encheva, Ilaria Gori, Rebecca E. Saunders, Rachael Instrell, Ozan Aygün, Marta Rodriguez-Martinez, Juston C. Weems, Gavin P. Kelly, Joan W. Conaway, Ronald C. Conaway, Aengus Stewart, Michael Howell, Ambrosius P. Snijders, Jesper Q. Svejstrup

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

Abstract

In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.

Original language	English (US)
Pages (from-to)	1597-1610
Number of pages	14
Journal	Cell Reports
Volume	15
Issue number	7
DOIs	https://doi.org/10.1016/j.celrep.2016.04.047
State	Published - May 17 2016
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/j.celrep.2016.04.047

Cite this

Boeing, S., Williamson, L., Encheva, V., Gori, I., Saunders, R. E., Instrell, R., Aygün, O., Rodriguez-Martinez, M., Weems, J. C., Kelly, G. P., Conaway, J. W., Conaway, R. C., Stewart, A., Howell, M., Snijders, A. P., & Svejstrup, J. Q. (2016). Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Reports, 15(7), 1597-1610. https://doi.org/10.1016/j.celrep.2016.04.047

@article{51b99936bf2e4dcc941580a8ec741c3e,

title = "Multiomic Analysis of the UV-Induced DNA Damage Response",

abstract = "In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.",

author = "Stefan Boeing and Laura Williamson and Vesela Encheva and Ilaria Gori and Saunders, {Rebecca E.} and Rachael Instrell and Ozan Ayg{\"u}n and Marta Rodriguez-Martinez and Weems, {Juston C.} and Kelly, {Gavin P.} and Conaway, {Joan W.} and Conaway, {Ronald C.} and Aengus Stewart and Michael Howell and Snijders, {Ambrosius P.} and Svejstrup, {Jesper Q.}",

note = "Publisher Copyright: {\textcopyright} 2016 The Author(s).",

year = "2016",

month = may,

day = "17",

doi = "10.1016/j.celrep.2016.04.047",

language = "English (US)",

volume = "15",

pages = "1597--1610",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "7",

}

TY - JOUR

T1 - Multiomic Analysis of the UV-Induced DNA Damage Response

AU - Boeing, Stefan

AU - Williamson, Laura

AU - Encheva, Vesela

AU - Gori, Ilaria

AU - Saunders, Rebecca E.

AU - Instrell, Rachael

AU - Aygün, Ozan

AU - Rodriguez-Martinez, Marta

AU - Weems, Juston C.

AU - Kelly, Gavin P.

AU - Conaway, Joan W.

AU - Conaway, Ronald C.

AU - Stewart, Aengus

AU - Howell, Michael

AU - Snijders, Ambrosius P.

AU - Svejstrup, Jesper Q.

PY - 2016/5/17

Y1 - 2016/5/17

N2 - In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.

AB - In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.

UR - http://www.scopus.com/inward/record.url?scp=84966552371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84966552371&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2016.04.047

DO - 10.1016/j.celrep.2016.04.047

M3 - Article

C2 - 27184836

AN - SCOPUS:84966552371

SN - 2211-1247

VL - 15

SP - 1597

EP - 1610

JO - Cell Reports

JF - Cell Reports

IS - 7

ER -

Multiomic Analysis of the UV-Induced DNA Damage Response

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this