Multi drug resistance-1 (MDR1) expression in response to chronic diazinon exposure: An in vitro study on Caco-2 cells

Peiman Habibollahi; Mohammad Hossein Ghahremani; Ebrahim Azizi; Seyed Nasser Ostad

doi:10.1007/s00128-010-0158-y

Multi drug resistance-1 (MDR1) expression in response to chronic diazinon exposure: An in vitro study on Caco-2 cells

Peiman Habibollahi, Mohammad Hossein Ghahremani, Ebrahim Azizi, Seyed Nasser Ostad

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

To evaluate the effect of chronic exposure to diazinon in the gastrointestinal tract, Caco-2 cells were made resistant by growing in low concentrations of diazinon (0.02 μM) that was gradually increased to 20 μM within 4.5 months. Resistant cells showed significant higher growth in the presence of 15, 45 and 135 μM of diazinon (96.08, 81.80 and 65.16% of control) compared to parent cells (79.71, 71.76 and 29.50% of control, respectively; p < 0.05). P-glycoprotein (P-gp) expression increased significantly in resistant cells (P-gp to beta-actin ratio 0.586 for parent and 1.255 for resistant cells, respectively; p < 0.05) without any alteration in MDR-1 mRNA level (p > 0.05).

Original language	English (US)
Pages (from-to)	105-109
Number of pages	5
Journal	Bulletin of Environmental Contamination and Toxicology
Volume	86
Issue number	1
DOIs	https://doi.org/10.1007/s00128-010-0158-y
State	Published - Jan 2011
Externally published	Yes

Keywords

Caco-2
Diazinon
Multi drug resistance 1
P-glycoprotein

ASJC Scopus subject areas

Toxicology
Pollution
Health, Toxicology and Mutagenesis

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10.1007/s00128-010-0158-y

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title = "Multi drug resistance-1 (MDR1) expression in response to chronic diazinon exposure: An in vitro study on Caco-2 cells",

abstract = "To evaluate the effect of chronic exposure to diazinon in the gastrointestinal tract, Caco-2 cells were made resistant by growing in low concentrations of diazinon (0.02 μM) that was gradually increased to 20 μM within 4.5 months. Resistant cells showed significant higher growth in the presence of 15, 45 and 135 μM of diazinon (96.08, 81.80 and 65.16% of control) compared to parent cells (79.71, 71.76 and 29.50% of control, respectively; p < 0.05). P-glycoprotein (P-gp) expression increased significantly in resistant cells (P-gp to beta-actin ratio 0.586 for parent and 1.255 for resistant cells, respectively; p < 0.05) without any alteration in MDR-1 mRNA level (p > 0.05).",

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author = "Peiman Habibollahi and Ghahremani, {Mohammad Hossein} and Ebrahim Azizi and Ostad, {Seyed Nasser}",

year = "2011",

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AU - Azizi, Ebrahim

AU - Ostad, Seyed Nasser

PY - 2011/1

Y1 - 2011/1

N2 - To evaluate the effect of chronic exposure to diazinon in the gastrointestinal tract, Caco-2 cells were made resistant by growing in low concentrations of diazinon (0.02 μM) that was gradually increased to 20 μM within 4.5 months. Resistant cells showed significant higher growth in the presence of 15, 45 and 135 μM of diazinon (96.08, 81.80 and 65.16% of control) compared to parent cells (79.71, 71.76 and 29.50% of control, respectively; p < 0.05). P-glycoprotein (P-gp) expression increased significantly in resistant cells (P-gp to beta-actin ratio 0.586 for parent and 1.255 for resistant cells, respectively; p < 0.05) without any alteration in MDR-1 mRNA level (p > 0.05).

AB - To evaluate the effect of chronic exposure to diazinon in the gastrointestinal tract, Caco-2 cells were made resistant by growing in low concentrations of diazinon (0.02 μM) that was gradually increased to 20 μM within 4.5 months. Resistant cells showed significant higher growth in the presence of 15, 45 and 135 μM of diazinon (96.08, 81.80 and 65.16% of control) compared to parent cells (79.71, 71.76 and 29.50% of control, respectively; p < 0.05). P-glycoprotein (P-gp) expression increased significantly in resistant cells (P-gp to beta-actin ratio 0.586 for parent and 1.255 for resistant cells, respectively; p < 0.05) without any alteration in MDR-1 mRNA level (p > 0.05).

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KW - Multi drug resistance 1

KW - P-glycoprotein

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Multi drug resistance-1 (MDR1) expression in response to chronic diazinon exposure: An in vitro study on Caco-2 cells

Abstract

Keywords

ASJC Scopus subject areas

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