TY - JOUR
T1 - Mouse strains to study cold-inducible beige progenitors and beige adipocyte formation and function
AU - Berry, Daniel C.
AU - Jiang, Yuwei
AU - Graff, Jonathan M.
N1 - Funding Information:
We are grateful to Dr Luis Parada for the use of equipment. We thank Dr Pierre Chambon for the SMA-CreERT2 mouse strain. We thank Drs Bill Richardson and Sean Morrison for the PDGFRa-CreERT2 mice. We thank Drs Rhonda Bassel-Duby and Eric N. Olson for the Myogenin-Cre and UCP1-CreERT2 mouse strains. This study was supported by the National Institute of Health and the National Institute of Diabetes and Digestive and Kidney Disease grants (R01 DK066556, R01 DK064261 and R01 DK088220) to J.M.G. D.C.B. was supported by the National Heart, Blood and Lung (5T32HL007360-34), and by the National Institute of Diabetes and Digestive and Kidney Disease (1F32DK101153-01A1). Y.J. was supported by the American Heart Association (13POST14590008).
Publisher Copyright:
© 2016, Nature Publishing Group. All rights reserved.
PY - 2016/1/5
Y1 - 2016/1/5
N2 - Cold temperatures induce formation of beige adipocytes, which convert glucose and fatty acids to heat, and may increase energy expenditure, reduce adiposity and lower blood glucose. This therapeutic potential is unrealized, hindered by a dearth of genetic tools to fate map, track and manipulate beige progenitors and 'beiging'. Here we examined 12 Cre/inducible Cre mouse strains that mark adipocyte, muscle and mural lineages, three proposed beige origins. Among these mouse strains, only those that marked perivascular mural cells tracked the cold-induced beige lineage. Two SMA-based strains, SMA-CreERT2 and SMA-rtTA, fate mapped into the majority of cold-induced beige adipocytes and SMA-marked progenitors appeared essential for beiging. Disruption of the potential of the SMA-tracked progenitors to form beige adipocytes was accompanied by an inability to maintain body temperature and by hyperglycaemia. Thus, SMA-engineered mice may be useful to track and manipulate beige progenitors, beige adipocyte formation and function.
AB - Cold temperatures induce formation of beige adipocytes, which convert glucose and fatty acids to heat, and may increase energy expenditure, reduce adiposity and lower blood glucose. This therapeutic potential is unrealized, hindered by a dearth of genetic tools to fate map, track and manipulate beige progenitors and 'beiging'. Here we examined 12 Cre/inducible Cre mouse strains that mark adipocyte, muscle and mural lineages, three proposed beige origins. Among these mouse strains, only those that marked perivascular mural cells tracked the cold-induced beige lineage. Two SMA-based strains, SMA-CreERT2 and SMA-rtTA, fate mapped into the majority of cold-induced beige adipocytes and SMA-marked progenitors appeared essential for beiging. Disruption of the potential of the SMA-tracked progenitors to form beige adipocytes was accompanied by an inability to maintain body temperature and by hyperglycaemia. Thus, SMA-engineered mice may be useful to track and manipulate beige progenitors, beige adipocyte formation and function.
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U2 - 10.1038/ncomms10184
DO - 10.1038/ncomms10184
M3 - Article
C2 - 26729601
AN - SCOPUS:84953212703
SN - 2041-1723
VL - 7
JO - Nature Communications
JF - Nature Communications
M1 - 10184
ER -