@inbook{b9f4798a5f044c60b87668d4c53f5676,
title = "Monitoring Mitochondria Function in Ferroptosis",
abstract = "Ferroptosis is a type of regulated necrosis driven by uncontrolled membrane lipid peroxidation. Mitochondria, which are membrane-bound organelles present in almost all eukaryotic cells and play a central role in energy metabolism and various types of cell death, have a complicated role in ferroptosis. On one hand, mitochondrial-derived iron metabolism and reactive oxygen species (ROS) production may promote ferroptosis. On the other hand, mitochondria also possess a dihydroorotate dehydrogenase (DHODH)-dependent antioxidant system that detoxifies lipid peroxides. This chapter summarizes several methods, such as western blotting, immunofluorescence, cell viability assays, mitochondrial fluorescent probes, adenosine 5′-triphosphate (ATP) assay kits, mitochondrial respiration, and mitophagy tests, that may enable researchers to gain a deeper understanding of the dual role of mitochondria in ferroptosis.",
keywords = "Ferroptosis, Methods, Mitochondria",
author = "Fangquan Chen and Jiao Liu and Daolin Tang and Rui Kang",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3433-2_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "103--115",
booktitle = "Methods in Molecular Biology",
}