Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression

Anna G. Sorace; Reshu Saini; Marshall Mahoney; Jason M. Warram; Kurt R. Zinn; Kenneth Hoyt

doi:10.1109/ULTSYM.2012.0512

Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression

Anna G. Sorace, Reshu Saini, Marshall Mahoney, Jason M. Warram, Kurt R. Zinn, Kenneth Hoyt

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Molecular ultrasound (US) imaging of overexpressed endothelial receptors has been shown to increase tumor visualization within a region-of-interest and is of particular interest in cancer research. This study used an in vivo animal model system for evaluating targeted microbubble (MB) contrast agents. 2LMP breast cancer-bearing mice (N = 6) expressed varying intratumoral levels of the target hemagglutinin (HA) receptor through adenoviral (Ad) vector modulation. Animals were administered a 'low' and then a 'high' Ad vector dose following initial US imaging. Anti-HA antibody-labeled or control MBs were prepared and molecular US imaging was performed 24 hours after Ad vector infection using a SONIX Tablet ultrasound system (Ultrasonix Medical Corp). Custom Matlab programs were developed to evaluate US image intensity from data collected using a MB destruction-replenishment technique. Results showed intratumoral enhancement due to targeted MB accumulation was significantly increased in the 'high' Ad vector dosed animals compared to the same animals administered a 'low' Ad vector dose (P = 0.001). Control MBs showed no significant differences between imaging days (P = 0.96). Additionally, targeted MBs on average produced a 10.5-fold increase in intratumoral image intensity for the 'low' HA receptor group and an 18.8-fold increase in image intensity for the 'high' HA receptor group compared to control MBs.

Original language	English (US)
Title of host publication	2012 IEEE International Ultrasonics Symposium, IUS 2012
Pages	2049-2051
Number of pages	3
DOIs	https://doi.org/10.1109/ULTSYM.2012.0512
State	Published - Dec 1 2012
Event	2012 IEEE International Ultrasonics Symposium, IUS 2012 - Dresden, Germany Duration: Oct 7 2012 → Oct 10 2012

Publication series

Name	IEEE International Ultrasonics Symposium, IUS
ISSN (Print)	1948-5719
ISSN (Electronic)	1948-5727

Other

Other	2012 IEEE International Ultrasonics Symposium, IUS 2012
Country/Territory	Germany
City	Dresden
Period	10/7/12 → 10/10/12

ASJC Scopus subject areas

Acoustics and Ultrasonics

Access to Document

10.1109/ULTSYM.2012.0512

Cite this

Sorace, A. G., Saini, R., Mahoney, M., Warram, J. M., Zinn, K. R., & Hoyt, K. (2012). Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression. In 2012 IEEE International Ultrasonics Symposium, IUS 2012 (pp. 2049-2051). Article 6562192 (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2012.0512

Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression. / Sorace, Anna G.; Saini, Reshu; Mahoney, Marshall et al.
2012 IEEE International Ultrasonics Symposium, IUS 2012. 2012. p. 2049-2051 6562192 (IEEE International Ultrasonics Symposium, IUS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sorace, AG, Saini, R, Mahoney, M, Warram, JM, Zinn, KR & Hoyt, K 2012, Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression. in 2012 IEEE International Ultrasonics Symposium, IUS 2012., 6562192, IEEE International Ultrasonics Symposium, IUS, pp. 2049-2051, 2012 IEEE International Ultrasonics Symposium, IUS 2012, Dresden, Germany, 10/7/12. https://doi.org/10.1109/ULTSYM.2012.0512

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abstract = "Molecular ultrasound (US) imaging of overexpressed endothelial receptors has been shown to increase tumor visualization within a region-of-interest and is of particular interest in cancer research. This study used an in vivo animal model system for evaluating targeted microbubble (MB) contrast agents. 2LMP breast cancer-bearing mice (N = 6) expressed varying intratumoral levels of the target hemagglutinin (HA) receptor through adenoviral (Ad) vector modulation. Animals were administered a 'low' and then a 'high' Ad vector dose following initial US imaging. Anti-HA antibody-labeled or control MBs were prepared and molecular US imaging was performed 24 hours after Ad vector infection using a SONIX Tablet ultrasound system (Ultrasonix Medical Corp). Custom Matlab programs were developed to evaluate US image intensity from data collected using a MB destruction-replenishment technique. Results showed intratumoral enhancement due to targeted MB accumulation was significantly increased in the 'high' Ad vector dosed animals compared to the same animals administered a 'low' Ad vector dose (P = 0.001). Control MBs showed no significant differences between imaging days (P = 0.96). Additionally, targeted MBs on average produced a 10.5-fold increase in intratumoral image intensity for the 'low' HA receptor group and an 18.8-fold increase in image intensity for the 'high' HA receptor group compared to control MBs.",

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AB - Molecular ultrasound (US) imaging of overexpressed endothelial receptors has been shown to increase tumor visualization within a region-of-interest and is of particular interest in cancer research. This study used an in vivo animal model system for evaluating targeted microbubble (MB) contrast agents. 2LMP breast cancer-bearing mice (N = 6) expressed varying intratumoral levels of the target hemagglutinin (HA) receptor through adenoviral (Ad) vector modulation. Animals were administered a 'low' and then a 'high' Ad vector dose following initial US imaging. Anti-HA antibody-labeled or control MBs were prepared and molecular US imaging was performed 24 hours after Ad vector infection using a SONIX Tablet ultrasound system (Ultrasonix Medical Corp). Custom Matlab programs were developed to evaluate US image intensity from data collected using a MB destruction-replenishment technique. Results showed intratumoral enhancement due to targeted MB accumulation was significantly increased in the 'high' Ad vector dosed animals compared to the same animals administered a 'low' Ad vector dose (P = 0.001). Control MBs showed no significant differences between imaging days (P = 0.96). Additionally, targeted MBs on average produced a 10.5-fold increase in intratumoral image intensity for the 'low' HA receptor group and an 18.8-fold increase in image intensity for the 'high' HA receptor group compared to control MBs.

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Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression

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