Molecular cloning and nucleotide sequences of cDNA clones of sheep and goat adrenocortical cytochromes P450_scc (CYP11A1)

We isolated cDNA clones of the mRNAs for cytochromes P450_scc (CYP11A1) from sheep and goat adrenocortices using the RT-PCR method. We determined the complete nucleotide sequences of the coding and 3′-flanking regions of these cDNA clones. The results confirmed the amino terminal sequence of the sheep cytochrome P450_scc reported previously [Miyatake et al., Biochim. Biophys. Acta 1215, 176-182 (1994)]. On the basis of comparison of the deduced amino acid sequences of various animals and rainbow trout cytochromes P450_scc, and other mitochondrial cytochrome P450 isozymes, we discussed the substrate-associated and adreno-ferredoxin-binding region of cytochrome P450_scc.