Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.

W. A. Weiss; E. C. Friedberg

doi:10.1002/j.1460-2075.1985.tb03819.x

Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.

W. A. Weiss, E. C. Friedberg

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A plasmid designated pNF101 was isolated by transforming rad10 mutants with a yeast genomic library and screening transformed cells for enhanced resistance to killing by u.v. radiation. Plasmid pNF101 fully complements the u.v. sensitivity of rad10 mutant strains and was shown to contain the RAD10 gene by genetic analysis of integrant strains. The nucleotide sequence of the RAD10 gene was determined. The coding region consists of 195 codons and could encode a polypeptide of calculated mol. wt. 22 616 daltons. RAD10 protein expressed in Escherichia coli maxicells has a mol. wt of approximately 30 kd measured by gel electrophoresis. The RAD10 gene was localized to chromosome XIII of Saccharomyces cerevisiae by hybridization of the cloned gene to yeast chromosomes resolved by electrophoresis, and by genetic analysis.

Original language	English (US)
Pages (from-to)	1575-1582
Number of pages	8
Journal	The EMBO journal
Volume	4
Issue number	6
DOIs	https://doi.org/10.1002/j.1460-2075.1985.tb03819.x
State	Published - Jun 1985

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

Access to Document

10.1002/j.1460-2075.1985.tb03819.x

Cite this

@article{ddd7afc019c046eb94fbf81c3e423ad0,

title = "Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.",

abstract = "A plasmid designated pNF101 was isolated by transforming rad10 mutants with a yeast genomic library and screening transformed cells for enhanced resistance to killing by u.v. radiation. Plasmid pNF101 fully complements the u.v. sensitivity of rad10 mutant strains and was shown to contain the RAD10 gene by genetic analysis of integrant strains. The nucleotide sequence of the RAD10 gene was determined. The coding region consists of 195 codons and could encode a polypeptide of calculated mol. wt. 22 616 daltons. RAD10 protein expressed in Escherichia coli maxicells has a mol. wt of approximately 30 kd measured by gel electrophoresis. The RAD10 gene was localized to chromosome XIII of Saccharomyces cerevisiae by hybridization of the cloned gene to yeast chromosomes resolved by electrophoresis, and by genetic analysis.",

author = "Weiss, {W. A.} and Friedberg, {E. C.}",

year = "1985",

month = jun,

doi = "10.1002/j.1460-2075.1985.tb03819.x",

language = "English (US)",

volume = "4",

pages = "1575--1582",

journal = "The EMBO journal",

issn = "0261-4189",

publisher = "Nature Publishing Group",

number = "6",

}

TY - JOUR

T1 - Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.

AU - Weiss, W. A.

AU - Friedberg, E. C.

PY - 1985/6

Y1 - 1985/6

N2 - A plasmid designated pNF101 was isolated by transforming rad10 mutants with a yeast genomic library and screening transformed cells for enhanced resistance to killing by u.v. radiation. Plasmid pNF101 fully complements the u.v. sensitivity of rad10 mutant strains and was shown to contain the RAD10 gene by genetic analysis of integrant strains. The nucleotide sequence of the RAD10 gene was determined. The coding region consists of 195 codons and could encode a polypeptide of calculated mol. wt. 22 616 daltons. RAD10 protein expressed in Escherichia coli maxicells has a mol. wt of approximately 30 kd measured by gel electrophoresis. The RAD10 gene was localized to chromosome XIII of Saccharomyces cerevisiae by hybridization of the cloned gene to yeast chromosomes resolved by electrophoresis, and by genetic analysis.

AB - A plasmid designated pNF101 was isolated by transforming rad10 mutants with a yeast genomic library and screening transformed cells for enhanced resistance to killing by u.v. radiation. Plasmid pNF101 fully complements the u.v. sensitivity of rad10 mutant strains and was shown to contain the RAD10 gene by genetic analysis of integrant strains. The nucleotide sequence of the RAD10 gene was determined. The coding region consists of 195 codons and could encode a polypeptide of calculated mol. wt. 22 616 daltons. RAD10 protein expressed in Escherichia coli maxicells has a mol. wt of approximately 30 kd measured by gel electrophoresis. The RAD10 gene was localized to chromosome XIII of Saccharomyces cerevisiae by hybridization of the cloned gene to yeast chromosomes resolved by electrophoresis, and by genetic analysis.

UR - http://www.scopus.com/inward/record.url?scp=0022083961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022083961&partnerID=8YFLogxK

U2 - 10.1002/j.1460-2075.1985.tb03819.x

DO - 10.1002/j.1460-2075.1985.tb03819.x

M3 - Article

C2 - 3896774

AN - SCOPUS:0022083961

SN - 0261-4189

VL - 4

SP - 1575

EP - 1582

JO - The EMBO journal

JF - The EMBO journal

IS - 6

ER -

Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this