TY - JOUR
T1 - Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates
T2 - Implications for the Cori cycle
AU - Garcia, Christine Kim
AU - Goldstein, Joseph L.
AU - Pathak, Ravindra K.
AU - Anderson, Richard G W
AU - Brown, Michael S.
N1 - Funding Information:
We thank Drs. Dennis Stone and Shmuel Muallem for helpful discussions; Tracye Martin for invaluable assistance with the tissue culture experiments; and Marc Duderstadt and Charlene Ahn for excellent technical assistance. This work was supported by research grants from the National Institutes of Health (HL20948) and the Perot Family Foundation. C. K. G. issupported by Medical ScientistsTraining Grant GM08014 and National Research Service Award GM07062 from the National Institutes of Health.
PY - 1994/3/11
Y1 - 1994/3/11
N2 - Lactate and pyruvate cross cell membranes via a monocarboxylate transporter (MCT) with well-defined properties but undefined molecular structure. We report the cloning of a cDNA encoding MCT1, a monocarboxylate transporter whose properties resemble those of the erythrocyte MCT, including proton symport, trans acceleration, and sensitivity to α-cyanocinammates. A Phe to Cys substitution in MCT1 converts it to Mev, a mevalonate transporter. MCT1 is abundant in erythrocytes, cardiac muscle, and basolateral intestinal epithelium. In skeletal muscle it is restricted to mitochondria-rich myocytes. As sperm traverse the epididymis, MCT1 switches from sperm to epithelial cells. MCT1 is present at low levels in liver, suggesting another MCT in this tissue. By exporting lactate from intestine and erythrocytes, MCT1 participates in the Cori cycle. It also participates in novel pathways of monocarboxylate metabolism in muscle and sperm.
AB - Lactate and pyruvate cross cell membranes via a monocarboxylate transporter (MCT) with well-defined properties but undefined molecular structure. We report the cloning of a cDNA encoding MCT1, a monocarboxylate transporter whose properties resemble those of the erythrocyte MCT, including proton symport, trans acceleration, and sensitivity to α-cyanocinammates. A Phe to Cys substitution in MCT1 converts it to Mev, a mevalonate transporter. MCT1 is abundant in erythrocytes, cardiac muscle, and basolateral intestinal epithelium. In skeletal muscle it is restricted to mitochondria-rich myocytes. As sperm traverse the epididymis, MCT1 switches from sperm to epithelial cells. MCT1 is present at low levels in liver, suggesting another MCT in this tissue. By exporting lactate from intestine and erythrocytes, MCT1 participates in the Cori cycle. It also participates in novel pathways of monocarboxylate metabolism in muscle and sperm.
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U2 - 10.1016/0092-8674(94)90361-1
DO - 10.1016/0092-8674(94)90361-1
M3 - Article
C2 - 8124722
AN - SCOPUS:0028294023
SN - 0092-8674
VL - 76
SP - 865
EP - 873
JO - Cell
JF - Cell
IS - 5
ER -