Molecular architecture of proliferative lupus nephritis as elucidated using 50-plex imaging mass cytometry proteomics

Anto Sam Crosslee Louis Sam Titus; Ying Tan; Phuongthy Tran; Julius Lindblom; Maryann Ivbievbiokun; Yitian Xu; Junjun Zheng; Ioannis Parodis; Qi Cai; Anthony Chang; Shu Hsia Chen; Minghui Zhao; Chandra Mohan

doi:10.1016/j.clim.2023.109713

Molecular architecture of proliferative lupus nephritis as elucidated using 50-plex imaging mass cytometry proteomics

Anto Sam Crosslee Louis Sam Titus, Ying Tan, Phuongthy Tran, Julius Lindblom, Maryann Ivbievbiokun, Yitian Xu, Junjun Zheng, Ioannis Parodis, Qi Cai, Anthony Chang, Shu Hsia Chen, Minghui Zhao, Chandra Mohan

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Due to unique advantages that allow high-dimensional tissue profiling, we postulated imaging mass cytometry (IMC) may shed novel insights on the molecular makeup of proliferative lupus nephritis (LN). This study interrogates the spatial expression profiles of 50 target proteins in LN and control kidneys. Proliferative LN glomeruli are marked by podocyte loss with immune infiltration dominated by CD45RO+, HLA-DR+ memory CD4 and CD8 T-cells, and CD163+ macrophages, with similar changes in tubulointerstitial regions. Macrophages are the predominant HLA-DR expressing antigen presenting cells with little expression elsewhere, while macrophages and T-cells predominate cellular crescents. End-stage sclerotic glomeruli are encircled by an acellular fibro-epithelial Bowman's space surrounded by immune infiltrates, all enmeshed in fibronectin. Proliferative LN also shows signs indicative of epithelial to mesenchymal plasticity of tubular cells and parietal epithelial cells. IMC enabled proteomics is a powerful tool to delineate the spatial architecture of LN at the protein level.

Original language	English (US)
Article number	109713
Journal	Clinical Immunology
Volume	254
DOIs	https://doi.org/10.1016/j.clim.2023.109713
State	Published - Sep 2023
Externally published	Yes

Keywords

IMC
Imaging mass cytometry
Imaging mass cytometry proteomics
Kidney imaging mass cytometry
Lupus nephritis
Multiplexed imaging of human kidney
Proliferative LN

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/j.clim.2023.109713

Cite this

Louis Sam Titus, A. S. C., Tan, Y., Tran, P., Lindblom, J., Ivbievbiokun, M., Xu, Y., Zheng, J., Parodis, I., Cai, Q., Chang, A., Chen, S. H., Zhao, M., & Mohan, C. (2023). Molecular architecture of proliferative lupus nephritis as elucidated using 50-plex imaging mass cytometry proteomics. Clinical Immunology, 254, Article 109713. https://doi.org/10.1016/j.clim.2023.109713

@article{5014ce59e017498f891fb1932d9c3dc2,

title = "Molecular architecture of proliferative lupus nephritis as elucidated using 50-plex imaging mass cytometry proteomics",

abstract = "Due to unique advantages that allow high-dimensional tissue profiling, we postulated imaging mass cytometry (IMC) may shed novel insights on the molecular makeup of proliferative lupus nephritis (LN). This study interrogates the spatial expression profiles of 50 target proteins in LN and control kidneys. Proliferative LN glomeruli are marked by podocyte loss with immune infiltration dominated by CD45RO+, HLA-DR+ memory CD4 and CD8 T-cells, and CD163+ macrophages, with similar changes in tubulointerstitial regions. Macrophages are the predominant HLA-DR expressing antigen presenting cells with little expression elsewhere, while macrophages and T-cells predominate cellular crescents. End-stage sclerotic glomeruli are encircled by an acellular fibro-epithelial Bowman's space surrounded by immune infiltrates, all enmeshed in fibronectin. Proliferative LN also shows signs indicative of epithelial to mesenchymal plasticity of tubular cells and parietal epithelial cells. IMC enabled proteomics is a powerful tool to delineate the spatial architecture of LN at the protein level.",

keywords = "IMC, Imaging mass cytometry, Imaging mass cytometry proteomics, Kidney imaging mass cytometry, Lupus nephritis, Multiplexed imaging of human kidney, Proliferative LN",

author = "{Louis Sam Titus}, {Anto Sam Crosslee} and Ying Tan and Phuongthy Tran and Julius Lindblom and Maryann Ivbievbiokun and Yitian Xu and Junjun Zheng and Ioannis Parodis and Qi Cai and Anthony Chang and Chen, {Shu Hsia} and Minghui Zhao and Chandra Mohan",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2023",

month = sep,

doi = "10.1016/j.clim.2023.109713",

language = "English (US)",

volume = "254",

journal = "Clinical Immunology",

issn = "1521-6616",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Molecular architecture of proliferative lupus nephritis as elucidated using 50-plex imaging mass cytometry proteomics

AU - Louis Sam Titus, Anto Sam Crosslee

AU - Tan, Ying

AU - Tran, Phuongthy

AU - Lindblom, Julius

AU - Ivbievbiokun, Maryann

AU - Xu, Yitian

AU - Zheng, Junjun

AU - Parodis, Ioannis

AU - Cai, Qi

AU - Chang, Anthony

AU - Chen, Shu Hsia

AU - Zhao, Minghui

AU - Mohan, Chandra

PY - 2023/9

Y1 - 2023/9

N2 - Due to unique advantages that allow high-dimensional tissue profiling, we postulated imaging mass cytometry (IMC) may shed novel insights on the molecular makeup of proliferative lupus nephritis (LN). This study interrogates the spatial expression profiles of 50 target proteins in LN and control kidneys. Proliferative LN glomeruli are marked by podocyte loss with immune infiltration dominated by CD45RO+, HLA-DR+ memory CD4 and CD8 T-cells, and CD163+ macrophages, with similar changes in tubulointerstitial regions. Macrophages are the predominant HLA-DR expressing antigen presenting cells with little expression elsewhere, while macrophages and T-cells predominate cellular crescents. End-stage sclerotic glomeruli are encircled by an acellular fibro-epithelial Bowman's space surrounded by immune infiltrates, all enmeshed in fibronectin. Proliferative LN also shows signs indicative of epithelial to mesenchymal plasticity of tubular cells and parietal epithelial cells. IMC enabled proteomics is a powerful tool to delineate the spatial architecture of LN at the protein level.

AB - Due to unique advantages that allow high-dimensional tissue profiling, we postulated imaging mass cytometry (IMC) may shed novel insights on the molecular makeup of proliferative lupus nephritis (LN). This study interrogates the spatial expression profiles of 50 target proteins in LN and control kidneys. Proliferative LN glomeruli are marked by podocyte loss with immune infiltration dominated by CD45RO+, HLA-DR+ memory CD4 and CD8 T-cells, and CD163+ macrophages, with similar changes in tubulointerstitial regions. Macrophages are the predominant HLA-DR expressing antigen presenting cells with little expression elsewhere, while macrophages and T-cells predominate cellular crescents. End-stage sclerotic glomeruli are encircled by an acellular fibro-epithelial Bowman's space surrounded by immune infiltrates, all enmeshed in fibronectin. Proliferative LN also shows signs indicative of epithelial to mesenchymal plasticity of tubular cells and parietal epithelial cells. IMC enabled proteomics is a powerful tool to delineate the spatial architecture of LN at the protein level.

KW - IMC

KW - Imaging mass cytometry

KW - Imaging mass cytometry proteomics

KW - Kidney imaging mass cytometry

KW - Lupus nephritis

KW - Multiplexed imaging of human kidney

KW - Proliferative LN

UR - http://www.scopus.com/inward/record.url?scp=85166588942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85166588942&partnerID=8YFLogxK

U2 - 10.1016/j.clim.2023.109713

DO - 10.1016/j.clim.2023.109713

M3 - Article

C2 - 37516396

AN - SCOPUS:85166588942

SN - 1521-6616

VL - 254

JO - Clinical Immunology

JF - Clinical Immunology

M1 - 109713

ER -

Molecular architecture of proliferative lupus nephritis as elucidated using 50-plex imaging mass cytometry proteomics

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this