Mixed infection and strain diversity in congenital cytomegalovirus infection

Shannon A. Ross, Zdenek Novak, Sunil Pati, Raj Kumar Patro, Jennifer Blumenthal, Vishwanath R. Danthuluri, Amina Ahmed, Marian G. Michaels, Pablo J. Sánchez, David I. Bernstein, Robert W. Tolan, April L. Palmer, William J. Britt, Karen B. Fowler, Suresh B. Boppana

Research output: Contribution to journalArticlepeer-review

70 Scopus citations


Background. Cytomegalovirus (CMV), the most common cause of congenital infection, exhibits extensive genetic variability. We sought to determine whether multiple CMV strains can be transmitted to the fetus and to describe the distribution of genotypes in the saliva, urine, and blood. Methods. Study subjects consisted of a convenience sampling of 28 infants found to be CMV-positive on newborn screening as part of an ongoing study. Genotyping was performed on saliva specimens obtained during newborn screening and urine, saliva, and blood obtained at a later time point within the first 3 weeks of life. Results. Six (21.4%) of the 28 saliva samples obtained within the first 2 days of life contained >1 CMV genotype. Multiple CMV genotypes were found in 39% (5/13) of urine, saliva, and blood samples obtained within the first 3 weeks of life from 13 of the 28 newborns. There was no predominance of a CMV genotype at a specific site; however, 4 infants demonstrated distinct CMV strains in different compartments. Conclusions. Infection withmultipleCMV strains occurs in infants with congenitalCMV infection. The impact of intrauterine infection with multiple virus strains on the pathogenesis and long-term outcome remains to be elucidated.

Original languageEnglish (US)
Pages (from-to)1003-1007
Number of pages5
JournalJournal of Infectious Diseases
Issue number7
StatePublished - Oct 1 2011

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Mixed infection and strain diversity in congenital cytomegalovirus infection'. Together they form a unique fingerprint.

Cite this