Microrna-218 regulates vascular patterning by modulation of slit-robo signaling

Eric M. Small, Lillian B. Sutherland, Kartik N. Rajagopalan, Shusheng Wang, Eric N. Olson

Research output: Contribution to journalArticlepeer-review

128 Scopus citations


Rationale: Establishment of a functional vasculature requires the interconnection and remodeling of nascent blood vessels. Precise regulation of factors that influence endothelial cell migration and function is essential for these stereotypical vascular patterning events. The secreted Slit ligands and their Robo receptors constitute a critical signaling pathway controlling the directed migration of both neurons and vascular endothelial cells during embryonic development, but the mechanisms of their regulation are incompletely understood. Objective: To identify microRNAs regulating aspects of the Slit-Robo pathway and vascular patterning. Methods and Results: Here, we provide evidence that microRNA (miR)-218, which is encoded by an intron of the Slit genes, inhibits the expression of Robo1 and Robo2 and multiple components of the heparan sulfate biosynthetic pathway. Using in vitro and in vivo approaches, we demonstrate that miR-218 directly represses the expression of Robo1, Robo2, and glucuronyl C5-epimerase (GLCE), and that an intact miR-218-Slit-Robo regulatory network is essential for normal vascularization of the retina. Knockdown of miR-218 results in aberrant regulation of this signaling axis, abnormal endothelial cell migration, and reduced complexity of the retinal vasculature. Conclusions: Our findings link Slit gene expression to the posttranscriptional regulation of Robo receptors and heparan sulfate biosynthetic enzymes, allowing for precise control over vascular guidance cues influencing the organization of blood vessels during development.

Original languageEnglish (US)
Pages (from-to)1336-1344
Number of pages9
JournalCirculation research
Issue number11
StatePublished - Nov 26 2010


  • Angiogenesis
  • Developmental biology
  • Gene regulation

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine


Dive into the research topics of 'Microrna-218 regulates vascular patterning by modulation of slit-robo signaling'. Together they form a unique fingerprint.

Cite this