Micronucleation of human somatic cells with colcemid

Andrew H. Crenshaw; Jerry W. Shay; Leonard R. Murrell

doi:10.1007/BF01665884

Micronucleation of human somatic cells with colcemid

Andrew H. Crenshaw, Jerry W. Shay, Leonard R. Murrell

Research output: Contribution to journal › Article › peer-review

Abstract

A method is described for the production with colcemid of micronucleate cells from normal human fibroblasts (Detroit 550) and human aneuploid D98/AH₂ cells. The colcemid dose is titrated to a concentration that prevents cellular attachment to, and spreading on, the substrate following attempted mitosis. Washing out the drug with growth medium followed by incubation in drug-free medium for 12 to 24 h produces micronucleate cells. The percentage of micronucleation obtainable is 50 to 70% by treatment with 0.1 ug/ml colcemid for 24 h followed by incubation of floating blocked mitotic cells in drug-free growth medium.

Original language	English (US)
Pages (from-to)	185-187
Number of pages	3
Journal	Journal of Tissue Culture Methods
Volume	8
Issue number	4
DOIs	https://doi.org/10.1007/BF01665884
State	Published - Dec 1983

Keywords

colcemid
colchicine
human fibroblasts
micronucleation

ASJC Scopus subject areas

Anatomy
Pathology and Forensic Medicine

Access to Document

10.1007/BF01665884

Cite this

@article{fcbff8584391402cbd6361d2b91d7b68,

title = "Micronucleation of human somatic cells with colcemid",

abstract = "A method is described for the production with colcemid of micronucleate cells from normal human fibroblasts (Detroit 550) and human aneuploid D98/AH2 cells. The colcemid dose is titrated to a concentration that prevents cellular attachment to, and spreading on, the substrate following attempted mitosis. Washing out the drug with growth medium followed by incubation in drug-free medium for 12 to 24 h produces micronucleate cells. The percentage of micronucleation obtainable is 50 to 70% by treatment with 0.1 ug/ml colcemid for 24 h followed by incubation of floating blocked mitotic cells in drug-free growth medium.",

keywords = "colcemid, colchicine, human fibroblasts, micronucleation",

author = "Crenshaw, {Andrew H.} and Shay, {Jerry W.} and Murrell, {Leonard R.}",

year = "1983",

month = dec,

doi = "10.1007/BF01665884",

language = "English (US)",

volume = "8",

pages = "185--187",

journal = "Journal of Tissue Culture Methods",

issn = "0271-8057",

publisher = "Springer Netherlands",

number = "4",

}

TY - JOUR

T1 - Micronucleation of human somatic cells with colcemid

AU - Crenshaw, Andrew H.

AU - Shay, Jerry W.

AU - Murrell, Leonard R.

PY - 1983/12

Y1 - 1983/12

N2 - A method is described for the production with colcemid of micronucleate cells from normal human fibroblasts (Detroit 550) and human aneuploid D98/AH2 cells. The colcemid dose is titrated to a concentration that prevents cellular attachment to, and spreading on, the substrate following attempted mitosis. Washing out the drug with growth medium followed by incubation in drug-free medium for 12 to 24 h produces micronucleate cells. The percentage of micronucleation obtainable is 50 to 70% by treatment with 0.1 ug/ml colcemid for 24 h followed by incubation of floating blocked mitotic cells in drug-free growth medium.

AB - A method is described for the production with colcemid of micronucleate cells from normal human fibroblasts (Detroit 550) and human aneuploid D98/AH2 cells. The colcemid dose is titrated to a concentration that prevents cellular attachment to, and spreading on, the substrate following attempted mitosis. Washing out the drug with growth medium followed by incubation in drug-free medium for 12 to 24 h produces micronucleate cells. The percentage of micronucleation obtainable is 50 to 70% by treatment with 0.1 ug/ml colcemid for 24 h followed by incubation of floating blocked mitotic cells in drug-free growth medium.

KW - colcemid

KW - colchicine

KW - human fibroblasts

KW - micronucleation

UR - http://www.scopus.com/inward/record.url?scp=0020931781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020931781&partnerID=8YFLogxK

U2 - 10.1007/BF01665884

DO - 10.1007/BF01665884

M3 - Article

AN - SCOPUS:0020931781

SN - 0271-8057

VL - 8

SP - 185

EP - 187

JO - Journal of Tissue Culture Methods

JF - Journal of Tissue Culture Methods

IS - 4

ER -

Micronucleation of human somatic cells with colcemid

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this