TY - GEN
T1 - Microfluidic microsphere-trap arrays for simultaneous detection of multiple targets
AU - Xu, Xiaoxiao
AU - Li, Zhenyu
AU - Kotagiri, Nalinikanth
AU - Sarder, Pinaki
AU - Achilefu, Samuel
AU - Nehorai, Arye
PY - 2013
Y1 - 2013
N2 - Microsphere arrays can be used to effectively detect, identify, and quantify biological targets, such as mRNAs, proteins, antibodies, and cells. In this work, we design a microfluidic microsphere-trap array device that enables simultaneous, efficient, and accurate screening of multiple targets on a single platform. Different types of targets are captured on the surfaces of microspheres of different sizes. By optimizing the geometric parameters of the traps, the trap arrays in this device can immobilize microspheres of different sizes at different regions with microfluidic hydrodynamic trapping. The targets are thus detected according to the microspheres' positions (position-encoding), which simplifies screening and avoids errors in target identification. We validate the design using fluid dynamics finite element simulations by COMSOL Multiphysics software using microsphere of two different sizes. We also performed preliminary microspheretrapping experiments on a fabricated device using microspheres of one size. Our results demonstrate that the proposed device can achieve the position-encoding of the microspheres with few fluidic errors. This device is promising for simultaneous detection of multiple targets and become a cheap and fast disease diagnostic tool.
AB - Microsphere arrays can be used to effectively detect, identify, and quantify biological targets, such as mRNAs, proteins, antibodies, and cells. In this work, we design a microfluidic microsphere-trap array device that enables simultaneous, efficient, and accurate screening of multiple targets on a single platform. Different types of targets are captured on the surfaces of microspheres of different sizes. By optimizing the geometric parameters of the traps, the trap arrays in this device can immobilize microspheres of different sizes at different regions with microfluidic hydrodynamic trapping. The targets are thus detected according to the microspheres' positions (position-encoding), which simplifies screening and avoids errors in target identification. We validate the design using fluid dynamics finite element simulations by COMSOL Multiphysics software using microsphere of two different sizes. We also performed preliminary microspheretrapping experiments on a fabricated device using microspheres of one size. Our results demonstrate that the proposed device can achieve the position-encoding of the microspheres with few fluidic errors. This device is promising for simultaneous detection of multiple targets and become a cheap and fast disease diagnostic tool.
KW - Microfluidics
KW - Microsphere-Trap Arrays
KW - Simultaneous Detection of Multiple Targets
UR - http://www.scopus.com/inward/record.url?scp=84878145682&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878145682&partnerID=8YFLogxK
U2 - 10.1117/12.2006628
DO - 10.1117/12.2006628
M3 - Conference contribution
AN - SCOPUS:84878145682
SN - 9780819493842
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Microfluidics, BioMEMS, and Medical Microsystems XI
T2 - Microfluidics, BioMEMS, and Medical Microsystems XI
Y2 - 3 February 2013 through 5 February 2013
ER -