TY - JOUR
T1 - Micellar lipid composition profoundly affects LXR-dependent cholesterol transport across CaCo2 cells
AU - Petruzzelli, Michele
AU - Groen, Albert K.
AU - van Erpecum, Karel J.
AU - Vrins, Carlos
AU - van der Velde, Astrid E.
AU - Portincasa, Piero
AU - Palasciano, Giuseppe
AU - van Berge Henegouwen, Gerard P.
AU - Sasso, Giuseppe Lo
AU - Morgano, Annalisa
AU - Moschetta, Antonio
PY - 2009/4/17
Y1 - 2009/4/17
N2 - Intraluminal phospholipids affect micellar solubilization and absorption of cholesterol. We here study cholesterol transport from taurocholate-phospholipid-cholesterol micelles to CaCo2 cells, and associated effects on ABC-A1 mediated cholesterol efflux. Micellar incorporation of egg-yolk-phosphatidylcholine markedly increased apical retention of the sterol with decreased expression of ABC-A1, an effect that is prevented by synthetic liver X receptor (LXR) or retinoid X receptor (RXR) agonists. On the other hand, incorporation of lyso-phosphatidylcholine (LysoPC) increased ABC-A1-HDL-dependent basolateral cholesterol efflux, an effect that is abated when LXR is silenced. Thus, the modulation of cholesterol metabolism via intraluminal phospholipids is related to the activity of the oxysterol nuclear receptor LXR.
AB - Intraluminal phospholipids affect micellar solubilization and absorption of cholesterol. We here study cholesterol transport from taurocholate-phospholipid-cholesterol micelles to CaCo2 cells, and associated effects on ABC-A1 mediated cholesterol efflux. Micellar incorporation of egg-yolk-phosphatidylcholine markedly increased apical retention of the sterol with decreased expression of ABC-A1, an effect that is prevented by synthetic liver X receptor (LXR) or retinoid X receptor (RXR) agonists. On the other hand, incorporation of lyso-phosphatidylcholine (LysoPC) increased ABC-A1-HDL-dependent basolateral cholesterol efflux, an effect that is abated when LXR is silenced. Thus, the modulation of cholesterol metabolism via intraluminal phospholipids is related to the activity of the oxysterol nuclear receptor LXR.
KW - ABC-A1
KW - Bile salt
KW - Intestine
KW - Liver X receptor
KW - Lyso-phosphatidylcholine
KW - Phosphatidylcholine
UR - http://www.scopus.com/inward/record.url?scp=64449087406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64449087406&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2009.03.021
DO - 10.1016/j.febslet.2009.03.021
M3 - Article
C2 - 19303409
AN - SCOPUS:64449087406
SN - 0014-5793
VL - 583
SP - 1274
EP - 1280
JO - FEBS Letters
JF - FEBS Letters
IS - 8
ER -