TY - CHAP
T1 - Methods for studying primary cilia in heart tissue after ischemia-reperfusion injury
AU - Kretschmar, Catalina
AU - Hernández-Cáceres, María Paz
AU - Reyes, Montserrat
AU - Peña-Oyarzún, Daniel
AU - García-Navarrete, Camila
AU - Troncoso, Rodrigo
AU - Díaz-Castro, Francisco
AU - Budini, Mauricio
AU - Morselli, Eugenia
AU - Riquelme, Jaime A.
AU - Hill, Joseph A.
AU - Lavandero, Sergio
AU - Criollo, Alfredo
N1 - Publisher Copyright:
© 2023 Elsevier Inc.
PY - 2023/1
Y1 - 2023/1
N2 - Cardiovascular diseases are the leading cause of death and disability worldwide. After heart injury triggered by myocardial ischemia or myocardial infarction, extensive zones of tissue are damaged and some of the tissue dies by necrosis and/or apoptosis. The loss of contractile mass activates a series of biochemical mechanisms that allow, through cardiac remodeling, the replacement of the dysfunctional heart tissue by fibrotic material. Our previous studies have shown that primary cilia, non-motile antenna-like structures at the cell surface required for the activation of specific signaling pathways, are present in cardiac fibroblasts and required for cardiac fibrosis induced by ischemia/reperfusion (I/R) in mice. I/R-induced myocardial fibrosis promotes the enrichment of ciliated cardiac fibroblasts where the myocardial injury occurs. Given discussions about the existence of cilia in specific cardiac cell types, as well as the functional relevance of studying cilia-dependent signaling in cardiac fibrosis after I/R, here we describe our methods to evaluate the presence and roles of primary cilia in cardiac fibrosis after I/R in mice.
AB - Cardiovascular diseases are the leading cause of death and disability worldwide. After heart injury triggered by myocardial ischemia or myocardial infarction, extensive zones of tissue are damaged and some of the tissue dies by necrosis and/or apoptosis. The loss of contractile mass activates a series of biochemical mechanisms that allow, through cardiac remodeling, the replacement of the dysfunctional heart tissue by fibrotic material. Our previous studies have shown that primary cilia, non-motile antenna-like structures at the cell surface required for the activation of specific signaling pathways, are present in cardiac fibroblasts and required for cardiac fibrosis induced by ischemia/reperfusion (I/R) in mice. I/R-induced myocardial fibrosis promotes the enrichment of ciliated cardiac fibroblasts where the myocardial injury occurs. Given discussions about the existence of cilia in specific cardiac cell types, as well as the functional relevance of studying cilia-dependent signaling in cardiac fibrosis after I/R, here we describe our methods to evaluate the presence and roles of primary cilia in cardiac fibrosis after I/R in mice.
KW - Cardiovascular diseases
KW - Fibrosis
KW - Heart
KW - Ischemia/reperfusion
KW - Primary cilia
UR - http://www.scopus.com/inward/record.url?scp=85146356203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85146356203&partnerID=8YFLogxK
U2 - 10.1016/bs.mcb.2022.12.013
DO - 10.1016/bs.mcb.2022.12.013
M3 - Chapter
C2 - 37164544
AN - SCOPUS:85146356203
SN - 9780443185885
T3 - Methods in Cell Biology
SP - 85
EP - 101
BT - Cilia
A2 - Bravo-San Pedro, José Manuel
A2 - Galluzzi, Lorenzo
PB - Academic Press Inc.
ER -