TY - JOUR
T1 - Methods for analysis of ligand-induced RNA conformational changes.
AU - Brautigam, Chad A
AU - Wakeman, Catherine A.
AU - Winkler, Wade C.
PY - 2009
Y1 - 2009
N2 - Encoded within many RNA sequences is the requisite information for folding of intricate three-dimensional structures. Moreover, many noncoding RNAs can adopt structurally distinct and functionally specialized conformations in response to specific cellular signals. These conformational transitions are often times accompanied by changes in hydrodynamic radii. Therefore, experimental methods that measure changes in hydrodynamic radius can be employed for study of signal-induced RNA conformational changes. Several hydrodynamic methods, including analytical ultracentrifugation, size-exclusion chromatography, and nondenaturing gel electrophoresis, are briefly discussed herein.
AB - Encoded within many RNA sequences is the requisite information for folding of intricate three-dimensional structures. Moreover, many noncoding RNAs can adopt structurally distinct and functionally specialized conformations in response to specific cellular signals. These conformational transitions are often times accompanied by changes in hydrodynamic radii. Therefore, experimental methods that measure changes in hydrodynamic radius can be employed for study of signal-induced RNA conformational changes. Several hydrodynamic methods, including analytical ultracentrifugation, size-exclusion chromatography, and nondenaturing gel electrophoresis, are briefly discussed herein.
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U2 - 10.1007/978-1-59745-558-9_7
DO - 10.1007/978-1-59745-558-9_7
M3 - Article
C2 - 19381554
AN - SCOPUS:65649113946
SN - 1064-3745
VL - 540
SP - 77
EP - 95
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -