TY - JOUR
T1 - Metabolism of [3-13c]-pyruvate by cysticerci of taenia crassiceps
AU - Corbin, I.
AU - Simcoff, R.
AU - Novak, M.
AU - Blackburn, B. J.
N1 - Funding Information:
Acknowledgement The financial assistance of NSERC (a grant to M.N. and B.J.B.) is gratefully acknowledged.
PY - 1998
Y1 - 1998
N2 - Carbon-13 decoupled 1H spin-echo nuclear magnetic resonance (NMR) spectra, with and without 13C population inversion, of extracts of Taenia crassiceps cysticerci incubated in media containing [3-13C]-pyruvate showed 13C enrichment in alanine, lactate, acetate, succinate, and citrate. Labeling in the latter three metabolites provides substantial evidence that the malic enzyme reaction in this cestode also functions in the direction opposite to that in which it is normally portrayed. The direct passage of pyruvate from the cytosol to the mitochondrion is suggested by the greater percentage of 13C detected in acetate relative to succinate.
AB - Carbon-13 decoupled 1H spin-echo nuclear magnetic resonance (NMR) spectra, with and without 13C population inversion, of extracts of Taenia crassiceps cysticerci incubated in media containing [3-13C]-pyruvate showed 13C enrichment in alanine, lactate, acetate, succinate, and citrate. Labeling in the latter three metabolites provides substantial evidence that the malic enzyme reaction in this cestode also functions in the direction opposite to that in which it is normally portrayed. The direct passage of pyruvate from the cytosol to the mitochondrion is suggested by the greater percentage of 13C detected in acetate relative to succinate.
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U2 - 10.1007/s004360050439
DO - 10.1007/s004360050439
M3 - Article
C2 - 9660145
AN - SCOPUS:0031596062
SN - 0044-3255
VL - 84
SP - 516
EP - 518
JO - Parasitology Research
JF - Parasitology Research
IS - 6
ER -