Abstract
Metabolic flux analysis of cellula growth is very important for the fermentation processes which have a small yield of metabolic products. A new analysis method is developed to calculate the least square solution of the intercellular metabolic flux distribution, which successfully combines cellular elemental composition data with cellular molecular composition data by incorporating carbon balance and generalized degree of reduction balance into the calculating process. This method can make the result more reliable, because it utilizes more measurement data and ensures data consistency. It should be mentioned that all results are derived from a simplified metabolic network of B. subtilis, which is created by representing the complex biomass synthesis network with some precursor requirement rates.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 847-852 |
| Number of pages | 6 |
| Journal | Huagong Xuebao/Journal of Chemical Industry and Engineering (China) |
| Volume | 53 |
| Issue number | 8 |
| State | Published - Aug 1 2002 |
Keywords
- B. subtilis
- Cell composition
- Cell growth process
- Metabolic flux analysis
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)