Membrane-buffer partition coefficients of semiquinones using the spin-broadening technique

Antonio E. Alegría; Sandra Rivera; Marilyn Hernández; Rafael Ufret; Mariely Morales

doi:10.1039/FT9938903773

Membrane-buffer partition coefficients of semiquinones using the spin-broadening technique

Antonio E. Alegría, Sandra Rivera, Marilyn Hernández, Rafael Ufret, Mariely Morales

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11 Scopus citations

Abstract

Membrane-buffer partition coefficients have been determined for 1,4-benzosemiquinone (BQ^.-), 1,4-naphthosemiquinone (NQ^.-) and naphthazarin semiquinone (NZQ^.-) in egg-yolk phosphatidylcholine (PC) multilamellar vesicles in sodium cacodylate buffer at pH 7.4, utilizing electron paramagnetic resonance (EPR) spectroscopy. Partition coefficients are in the order BQ^.- < NZQ^.- < NQ^.-, which is the same order followed by the octanol-water partition coefficients of their parent neutral quinones. A method is proposed for the determination of the fraction of semiquinones at the membrane which are located in the hydrophobic inner site. This fraction also follows the same order as the partition coefficients.

Original language	English (US)
Pages (from-to)	3773-3777
Number of pages	5
Journal	Journal of the Chemical Society, Faraday Transactions
Volume	89
Issue number	20
DOIs	https://doi.org/10.1039/FT9938903773
State	Published - 1993

ASJC Scopus subject areas

Physical and Theoretical Chemistry

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10.1039/FT9938903773

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title = "Membrane-buffer partition coefficients of semiquinones using the spin-broadening technique",

abstract = "Membrane-buffer partition coefficients have been determined for 1,4-benzosemiquinone (BQ.-), 1,4-naphthosemiquinone (NQ.-) and naphthazarin semiquinone (NZQ.-) in egg-yolk phosphatidylcholine (PC) multilamellar vesicles in sodium cacodylate buffer at pH 7.4, utilizing electron paramagnetic resonance (EPR) spectroscopy. Partition coefficients are in the order BQ.- < NZQ.- < NQ.-, which is the same order followed by the octanol-water partition coefficients of their parent neutral quinones. A method is proposed for the determination of the fraction of semiquinones at the membrane which are located in the hydrophobic inner site. This fraction also follows the same order as the partition coefficients.",

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T1 - Membrane-buffer partition coefficients of semiquinones using the spin-broadening technique

AU - Alegría, Antonio E.

AU - Rivera, Sandra

AU - Hernández, Marilyn

AU - Ufret, Rafael

AU - Morales, Mariely

PY - 1993

Y1 - 1993

N2 - Membrane-buffer partition coefficients have been determined for 1,4-benzosemiquinone (BQ.-), 1,4-naphthosemiquinone (NQ.-) and naphthazarin semiquinone (NZQ.-) in egg-yolk phosphatidylcholine (PC) multilamellar vesicles in sodium cacodylate buffer at pH 7.4, utilizing electron paramagnetic resonance (EPR) spectroscopy. Partition coefficients are in the order BQ.- < NZQ.- < NQ.-, which is the same order followed by the octanol-water partition coefficients of their parent neutral quinones. A method is proposed for the determination of the fraction of semiquinones at the membrane which are located in the hydrophobic inner site. This fraction also follows the same order as the partition coefficients.

AB - Membrane-buffer partition coefficients have been determined for 1,4-benzosemiquinone (BQ.-), 1,4-naphthosemiquinone (NQ.-) and naphthazarin semiquinone (NZQ.-) in egg-yolk phosphatidylcholine (PC) multilamellar vesicles in sodium cacodylate buffer at pH 7.4, utilizing electron paramagnetic resonance (EPR) spectroscopy. Partition coefficients are in the order BQ.- < NZQ.- < NQ.-, which is the same order followed by the octanol-water partition coefficients of their parent neutral quinones. A method is proposed for the determination of the fraction of semiquinones at the membrane which are located in the hydrophobic inner site. This fraction also follows the same order as the partition coefficients.

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Membrane-buffer partition coefficients of semiquinones using the spin-broadening technique

Abstract

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