@article{87d11a0143804537901eb728fddf6a6b,
title = "Measurement of CD74 N-terminal Fragment Accumulation in Cells Treated with SPPL2a Inhibitor",
abstract = "The recent discovery of human signal peptide peptidase-like 2a (SPPL2a) deficiency in humans revealed the toxicity associated with the accumulation of one of its substrates, CD74 N-terminal fragment (CD74-NTF), for certain type of dendritic cells (cDC2). We developed a two-step protocol for monitoring the accumulation of this molecule in different subsets of PBMCs and immortalized B cells, in which SPPL2a is chemically inhibited and CD74-NTF levels are then assessed by flow cytometry or western blotting. The chemical inhibition of SPPL2a has been described elsewhere, but this is the first time that this inhibition has been reported as a protocol.",
keywords = "458, CD74-NTF, Chemical, Inhibition, L-685, SPPL2a",
author = "Rub{\'e}n Mart{\'i}nez-Barricarte and Kong, {Xiao Fei} and Casanova, {Jean Laurent}",
note = "Funding Information: We thank all the authors of our original research article (Kong et al., 2018). R.M.B. was funded by a European Molecular Biology Organization (EMBO) long-term fellowship. X.-F.K. was supported by the Jerome Lejeune Foundation, the Stony Wold-Herbert Fund, the Choh-Hao Li Memorial Fund Scholar Award, and the Shanghai Educational Development Foundation. The Laboratory of Human Genetics of Infectious Diseases was supported by grants from the St. Giles Foundation (J.-L.C.), The Rockefeller University Center for Clinical and Translational Science (grant UL1TR001866 from the National Center for Research Resources and the National Center for Advancing Sciences (NCATS) to R.M.-B. and X.-F.K.), the National Institutes of Health, the National Institute of Allergy and Infectious Diseases (5R01AI089970-02 and 5R37AI095983 to J.-L.C.), Institut National de la Sant{\'e} et de la Recherche M{\'e}dicale (INSERM), Paris Descartes University, and The Rockefeller University. Publisher Copyright: Copyright {\textcopyright} 2019 The Authors; exclusive licensee Bio-protocol LLC.",
year = "2019",
month = jun,
day = "5",
doi = "10.21769/BioProtoc.3254",
language = "English (US)",
volume = "9",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "Bio-protocol LLC",
number = "11",
}