Marker for type VI secretion system effectors

Dor Salomon, Lisa N. Kinch, David C. Trudgian, Xiaofeng Guo, John A. Klimko, Nick V. Grishin, Hamid Mirzaei, Kim Orth

Research output: Contribution to journalArticlepeer-review

114 Scopus citations


Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic bacterial proteins that are primarily located in the T6SS genome neighborhood. We demonstrate that several MIX-containing proteins are T6SS effectors and that they are not required for T6SS activity. Thus, we propose that MIX-containing proteins are T6SS effectors. Our findings allow for the identification of numerous uncharacterized T6SS effectors that will undoubtedly lead to the discovery of new biological mechanisms.

Original languageEnglish (US)
Pages (from-to)9271-9276
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number25
StatePublished - Jun 24 2014


  • Bacterial competition
  • Colicin
  • Toxin
  • Vibrio

ASJC Scopus subject areas

  • General


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