Mapping of Marek's disease virus genome: Identification of junction sequences between unique and inverted repeat regions

Koichi Makimura; Fang Yu Peng; Miho Tsuji; Saori Hasegawa; Yuri Kawai; Meihan Nonoyama; Akiko Tanaka

doi:10.1007/BF01703598

Mapping of Marek's disease virus genome: Identification of junction sequences between unique and inverted repeat regions

Koichi Makimura, Fang Yu Peng, Miho Tsuji, Saori Hasegawa, Yuri Kawai, Meihan Nonoyama, Akiko Tanaka

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Polymerase chain reaction (PCR) to amplify MDV DNA and subsequent sequencing identified the junction of TR_L/U_L, U_L/IR^L, IR_S/U_S, and U_S/TR_S. The TR_L/U_L junction is located 192 bp downstream of the last EcoRI site in the TR_L region, while the U_L/IR_L junction is located 192 bp upstream of the first EcoRI restriction enzyme site in the IR_L region. The IR_S/U_S junction is located 950 bp downstream of the second EcoRI site in the IR_S region, while the U_S/TR_S junction is located 950 bp upstream of the first EcoRI restriction enzyme site in the TR_S region. BamHI restriction enzyme mapping of one of the PCR products identified two novel DNA subfragments, BamHI-U₂ and -P₄, upstream of the U_S/TR_S junction of the MDV genome. Sequencing of the BamHI-D fragment revealed a novel open reading frame (ORF) encoding a 155 amino acid protein. The TR_L/U_L junction is located in this ORF. The N-terminal 65 amino acids of this protein is homologous to the N-terminal region of the previously reported pp38, which is located in the U_L/IR_L region. Computer-assisted analysis indicated that both are transmembrane proteins and that they share an antigenic domain.

Original language	English (US)
Pages (from-to)	15-24
Number of pages	10
Journal	Virus Genes
Volume	8
Issue number	1
DOIs	https://doi.org/10.1007/BF01703598
State	Published - Jan 1994

Keywords

MDV BamHI mapping
junction of inverted repeat and unique region
pp38

ASJC Scopus subject areas

Molecular Biology
Genetics
Virology

Access to Document

10.1007/BF01703598

Cite this

@article{5b40e4de67fc4cb4981e86c2e69749f3,

title = "Mapping of Marek's disease virus genome: Identification of junction sequences between unique and inverted repeat regions",

abstract = "Polymerase chain reaction (PCR) to amplify MDV DNA and subsequent sequencing identified the junction of TRL/UL, UL/IRL, IRS/US, and US/TRS. The TRL/UL junction is located 192 bp downstream of the last EcoRI site in the TRL region, while the UL/IRL junction is located 192 bp upstream of the first EcoRI restriction enzyme site in the IRL region. The IRS/US junction is located 950 bp downstream of the second EcoRI site in the IRS region, while the US/TRS junction is located 950 bp upstream of the first EcoRI restriction enzyme site in the TRS region. BamHI restriction enzyme mapping of one of the PCR products identified two novel DNA subfragments, BamHI-U2 and -P4, upstream of the US/TRS junction of the MDV genome. Sequencing of the BamHI-D fragment revealed a novel open reading frame (ORF) encoding a 155 amino acid protein. The TRL/UL junction is located in this ORF. The N-terminal 65 amino acids of this protein is homologous to the N-terminal region of the previously reported pp38, which is located in the UL/IRL region. Computer-assisted analysis indicated that both are transmembrane proteins and that they share an antigenic domain.",

keywords = "MDV BamHI mapping, junction of inverted repeat and unique region, pp38",

author = "Koichi Makimura and Peng, {Fang Yu} and Miho Tsuji and Saori Hasegawa and Yuri Kawai and Meihan Nonoyama and Akiko Tanaka",

year = "1994",

month = jan,

doi = "10.1007/BF01703598",

language = "English (US)",

volume = "8",

pages = "15--24",

journal = "Virus Genes",

issn = "0920-8569",

publisher = "Springer Netherlands",

number = "1",

}

TY - JOUR

T1 - Mapping of Marek's disease virus genome

T2 - Identification of junction sequences between unique and inverted repeat regions

AU - Makimura, Koichi

AU - Peng, Fang Yu

AU - Tsuji, Miho

AU - Hasegawa, Saori

AU - Kawai, Yuri

AU - Nonoyama, Meihan

AU - Tanaka, Akiko

PY - 1994/1

Y1 - 1994/1

N2 - Polymerase chain reaction (PCR) to amplify MDV DNA and subsequent sequencing identified the junction of TRL/UL, UL/IRL, IRS/US, and US/TRS. The TRL/UL junction is located 192 bp downstream of the last EcoRI site in the TRL region, while the UL/IRL junction is located 192 bp upstream of the first EcoRI restriction enzyme site in the IRL region. The IRS/US junction is located 950 bp downstream of the second EcoRI site in the IRS region, while the US/TRS junction is located 950 bp upstream of the first EcoRI restriction enzyme site in the TRS region. BamHI restriction enzyme mapping of one of the PCR products identified two novel DNA subfragments, BamHI-U2 and -P4, upstream of the US/TRS junction of the MDV genome. Sequencing of the BamHI-D fragment revealed a novel open reading frame (ORF) encoding a 155 amino acid protein. The TRL/UL junction is located in this ORF. The N-terminal 65 amino acids of this protein is homologous to the N-terminal region of the previously reported pp38, which is located in the UL/IRL region. Computer-assisted analysis indicated that both are transmembrane proteins and that they share an antigenic domain.

AB - Polymerase chain reaction (PCR) to amplify MDV DNA and subsequent sequencing identified the junction of TRL/UL, UL/IRL, IRS/US, and US/TRS. The TRL/UL junction is located 192 bp downstream of the last EcoRI site in the TRL region, while the UL/IRL junction is located 192 bp upstream of the first EcoRI restriction enzyme site in the IRL region. The IRS/US junction is located 950 bp downstream of the second EcoRI site in the IRS region, while the US/TRS junction is located 950 bp upstream of the first EcoRI restriction enzyme site in the TRS region. BamHI restriction enzyme mapping of one of the PCR products identified two novel DNA subfragments, BamHI-U2 and -P4, upstream of the US/TRS junction of the MDV genome. Sequencing of the BamHI-D fragment revealed a novel open reading frame (ORF) encoding a 155 amino acid protein. The TRL/UL junction is located in this ORF. The N-terminal 65 amino acids of this protein is homologous to the N-terminal region of the previously reported pp38, which is located in the UL/IRL region. Computer-assisted analysis indicated that both are transmembrane proteins and that they share an antigenic domain.

KW - MDV BamHI mapping

KW - junction of inverted repeat and unique region

KW - pp38

UR - http://www.scopus.com/inward/record.url?scp=0028324173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028324173&partnerID=8YFLogxK

U2 - 10.1007/BF01703598

DO - 10.1007/BF01703598

M3 - Article

C2 - 8209419

AN - SCOPUS:0028324173

SN - 0920-8569

VL - 8

SP - 15

EP - 24

JO - Virus Genes

JF - Virus Genes

IS - 1

ER -

Mapping of Marek's disease virus genome: Identification of junction sequences between unique and inverted repeat regions

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this