Macrophage-mediated inflammatory response decreases mycobacterial survival in mouse MSCs by augmenting NO production

Kun Yang, Yongjian Wu, Heping Xie, Miao Li, Siqi Ming, Liyan Li, Meiyu Li, Minhao Wu, Sitang Gong, Xi Huang

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Mycobacterium tuberculosis (MTB) is a hard-to-eradicate intracellular microbe, which escapes host immune attack during latent infection. Recent studies reveal that mesenchymal stem cells (MSCs) provide a protective niche for MTB to maintain latency. However, the regulation of mycobacterial residency in MSCs in the infectious microenvironment remains largely unknown. Here, we found that macrophage-mediated inflammatory response during MTB infection facilitated the clearance of bacilli residing in mouse MSCs. Higher inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production were observed in mouse MSCs under macrophage-mediated inflammatory circumstance. Blocking NO production in MSCs increased the survival of intracellular mycobacteria, indicating NO-mediated antimycobacterial activity. Moreover, both nuclear factor κB (NF-κB) and Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathways were involved in iNOS expression and NO production in inflammatory microenvironment. Furthermore, pro-inflammatory cytokine interleukin-1β could trigger NO production in MSCs and exert anti-mycobacterial activity via NF-κB signaling pathway. Neutralization of interleukin-1β in macrophage-mediated inflammatory microenvironment dampened the ability of mouse MSCs to produce NO. Together, our findings demonstrated that macrophage-mediated inflammatory response during mycobacterial infection promotes the clearance of bacilli in mouse MSCs by increasing NO production, which may provide a better understanding of latent MTB infection.

Original languageEnglish (US)
Article number27326
JournalScientific reports
StatePublished - Jun 2 2016
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Macrophage-mediated inflammatory response decreases mycobacterial survival in mouse MSCs by augmenting NO production'. Together they form a unique fingerprint.

Cite this