TY - JOUR
T1 - Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography
AU - Medalia, Ohad
AU - Weber, Igor
AU - Frangakis, Achilleas S.
AU - Nicastro, Daniela
AU - Gerisch, Günther
AU - Baumeister, Wolfgang
PY - 2002/11/8
Y1 - 2002/11/8
N2 - Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.
AB - Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.
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U2 - 10.1126/science.1076184
DO - 10.1126/science.1076184
M3 - Article
C2 - 12424373
AN - SCOPUS:0037044862
SN - 0036-8075
VL - 298
SP - 1209
EP - 1213
JO - Science
JF - Science
IS - 5596
ER -