Live Mapping of the Brain Extracellular Matrix and Remodeling in Neurological Disorders

Xiaoqian Ge; Xueqi Xu; Qi Cai; Hejian Xiong; Chen Xie; Yi Hong; Xiaofei Gao; Yao Yao; Robert Bachoo; Zhenpeng Qin

doi:10.1002/smtd.202301117

Live Mapping of the Brain Extracellular Matrix and Remodeling in Neurological Disorders

Xiaoqian Ge, Xueqi Xu, Qi Cai, Hejian Xiong, Chen Xie, Yi Hong, Xiaofei Gao, Yao Yao, Robert Bachoo, Zhenpeng Qin

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Live imaging of the brain extracellular matrix (ECM) provides vital insights into changes that occur in neurological disorders. Current techniques such as second or third-harmonic generation offer limited contrast for live imaging of the brain ECM. Here, a new method, pan-ECM via chemical labeling of extracellular proteins, is introduced for live brain ECM imaging. pan-ECM labels all major ECM components in live tissue including the interstitial matrix, basement membrane, and perineuronal nets. pan-ECM enables in vivo observation of the ECM heterogeneity between the glioma core and margin, as well as the assessment of ECM deterioration under stroke condition, without ECM shrinkage from tissue fixation. These findings indicate that the pan-ECM approach is a novel way to image the entire brain ECM in live brain tissue with optical resolution. pan-ECM has the potential to advance the understanding of ECM in brain function and neurological diseases.

Original language	English (US)
Article number	2301117
Journal	Small Methods
Volume	8
Issue number	1
DOIs	https://doi.org/10.1002/smtd.202301117
State	Published - Jan 19 2024
Externally published	Yes

Keywords

brain extracellular matrix
brain extracellular space
glioblastoma
live tissue imaging
stroke

ASJC Scopus subject areas

General Chemistry
General Materials Science

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10.1002/smtd.202301117

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title = "Live Mapping of the Brain Extracellular Matrix and Remodeling in Neurological Disorders",

abstract = "Live imaging of the brain extracellular matrix (ECM) provides vital insights into changes that occur in neurological disorders. Current techniques such as second or third-harmonic generation offer limited contrast for live imaging of the brain ECM. Here, a new method, pan-ECM via chemical labeling of extracellular proteins, is introduced for live brain ECM imaging. pan-ECM labels all major ECM components in live tissue including the interstitial matrix, basement membrane, and perineuronal nets. pan-ECM enables in vivo observation of the ECM heterogeneity between the glioma core and margin, as well as the assessment of ECM deterioration under stroke condition, without ECM shrinkage from tissue fixation. These findings indicate that the pan-ECM approach is a novel way to image the entire brain ECM in live brain tissue with optical resolution. pan-ECM has the potential to advance the understanding of ECM in brain function and neurological diseases.",

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author = "Xiaoqian Ge and Xueqi Xu and Qi Cai and Hejian Xiong and Chen Xie and Yi Hong and Xiaofei Gao and Yao Yao and Robert Bachoo and Zhenpeng Qin",

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AU - Ge, Xiaoqian

AU - Xu, Xueqi

AU - Cai, Qi

AU - Xiong, Hejian

AU - Xie, Chen

AU - Hong, Yi

AU - Gao, Xiaofei

AU - Yao, Yao

AU - Bachoo, Robert

AU - Qin, Zhenpeng

PY - 2024/1/19

Y1 - 2024/1/19

N2 - Live imaging of the brain extracellular matrix (ECM) provides vital insights into changes that occur in neurological disorders. Current techniques such as second or third-harmonic generation offer limited contrast for live imaging of the brain ECM. Here, a new method, pan-ECM via chemical labeling of extracellular proteins, is introduced for live brain ECM imaging. pan-ECM labels all major ECM components in live tissue including the interstitial matrix, basement membrane, and perineuronal nets. pan-ECM enables in vivo observation of the ECM heterogeneity between the glioma core and margin, as well as the assessment of ECM deterioration under stroke condition, without ECM shrinkage from tissue fixation. These findings indicate that the pan-ECM approach is a novel way to image the entire brain ECM in live brain tissue with optical resolution. pan-ECM has the potential to advance the understanding of ECM in brain function and neurological diseases.

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KW - brain extracellular space

KW - glioblastoma

KW - live tissue imaging

KW - stroke

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Live Mapping of the Brain Extracellular Matrix and Remodeling in Neurological Disorders

Abstract

Keywords

ASJC Scopus subject areas

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