TY - JOUR
T1 - Liposome-cell interactions
T2 - In vitro discrimination of uptake mechanism and in vivo targeting strategies to mononuclear phagocytes
AU - Schroit, Alan J.
AU - Madsen, J.
AU - Nayar, R.
N1 - Funding Information:
There is increasing evidence that reticuloendothelial cells in general, and macrophages in particular, are of major importance in host defense against neoplasia [1-3]. This has stimulated an enormous amount of interest in the potential value of macrophage-activating agents and the selective delivery of these agents to macrophages as therapeutic modalities against neoplastic disease. Significant increases in the efficacy of a variety of macrophage-activating agents have been achieved with liposomes [3-8]. Indeed, because of their capacity to retain *This work was supported in part by Developmental Fund Grant 175416 from the University of Texas M.D. Anderson Hospital and Tumor Institute at Houston and by National Institutes of Health Grant CA-40149. R.N. is a postdoctoral fellow of the Medical Research Council of Canada.
PY - 1986
Y1 - 1986
N2 - The interactions of liposomes with cells have been extensively studied to determine their potential use as vehicles for the delivery of drugs in vivo. Since intravenously administered liposomes are, for the most part, cleared by cells of the reticuloendothelial system (RES), considerable effort has been made to take advantage of this phenomenon rather than view it as an obstacle. Indeed, cells of the RES, in particular macrophages, have been shown to play a vital role in homeostasis and in host defence mechanisms against infection and neoplasia. In this article, we present an overview of liposome-cell interactions, with particular emphasis on the techniques used to monitor the interaction of liposomes with macrophages. Specifically, we discuss methodologies which can be used to differentiate between liposome-cell fusion, adsorption and endocytosis in vitro. In addition, we outline the various strategies that have been employed for both actively and passively targeting liposomes to macrophages in vivo. We also review the rationale and various techniques for designing liposomes for enhanced macrophage uptake, which, in certain cases, results in the selective release of liposome-entrapped compounds in situ.
AB - The interactions of liposomes with cells have been extensively studied to determine their potential use as vehicles for the delivery of drugs in vivo. Since intravenously administered liposomes are, for the most part, cleared by cells of the reticuloendothelial system (RES), considerable effort has been made to take advantage of this phenomenon rather than view it as an obstacle. Indeed, cells of the RES, in particular macrophages, have been shown to play a vital role in homeostasis and in host defence mechanisms against infection and neoplasia. In this article, we present an overview of liposome-cell interactions, with particular emphasis on the techniques used to monitor the interaction of liposomes with macrophages. Specifically, we discuss methodologies which can be used to differentiate between liposome-cell fusion, adsorption and endocytosis in vitro. In addition, we outline the various strategies that have been employed for both actively and passively targeting liposomes to macrophages in vivo. We also review the rationale and various techniques for designing liposomes for enhanced macrophage uptake, which, in certain cases, results in the selective release of liposome-entrapped compounds in situ.
KW - drug release
KW - liposome-cell interactions
KW - liposomes
KW - mononuclear phagocytes
KW - targeting
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U2 - 10.1016/0009-3084(86)90080-0
DO - 10.1016/0009-3084(86)90080-0
M3 - Article
C2 - 3527460
AN - SCOPUS:0022480155
SN - 0009-3084
VL - 40
SP - 373
EP - 393
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
IS - 2-4
ER -