Light-sheet engineering using the Field Synthesis theorem

Bo Jui Chang, Reto P Fiolka

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Recent advances in light-sheet microscopy have enabled sensitive imaging with high spatiotemporal resolution. However, the creation of thin light-sheets for high axial resolution is challenging, as the thickness of the sheet, field of view and confinement of the excitation need to be carefully balanced. Some of the thinnest light-sheets created so far have found little practical use as they excite too much out-of-focus fluorescence. In contrast, the most commonly used light-sheet for subcellular imaging, the square lattice, has excellent excitation confinement at the cost of lower axial resolving power. Here we leverage the recently discovered Field Synthesis theorem to create light-sheets where thickness and illumination confinement can be continuously tuned. Explicitly, we scan a line beam across a portion of an annulus mask on the back focal plane of the illumination objective, which we call C-light-sheets. We experimentally characterize these light-sheets and demonstrate their application on biological samples.

Original languageEnglish (US)
Article number014001
JournalJPhys Photonics
Issue number1
StatePublished - Nov 14 2019


  • C-light-sheet
  • Field Synthesis
  • Fluorescence microscopy
  • Lattice light-sheet

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering


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