Abstract
Background: Persistent elevation of estrogens after injury or sepsis correlates with increased mortality and a pro-inflammatory state. Given that aromatase is elevated after injury, the enzyme's subsequent conversion of androstenedione and testosterone to estrone and estradiol may be a causative factor for this correlation. Methods: Aromatase knockout (ArKO) and wild type female mice were subjected to a 15% total body surface area burn. The delayed type hypersensitivity (DTH) response and splenocyte production of IL-6 and TNFα were examined 8 days later. Results: Injury in wild type mice is associated with an impairment in the DTH response, as well as with an increase in IL-6 and TNFα production by stimulated splenocytes. However, for ArKO mice, the impairment in DTH was blunted and there was no difference in IL-6 production between sham- and burn-injured mice. Sham-injured ArKO mice produced nearly 50% more TNFα than wild type mice, while injury did not result in a significant increase in TNFα production for ArKO mice. Conclusion: The complete deficiency in aromatase correlated with a decrease in the production of the inflammatory cytokine IL-6 and partial restoration of the DTH response after severe burn. However, a deficiency of aromatase did not effect TNFα production after injury.
Original language | English (US) |
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Pages (from-to) | 577-582 |
Number of pages | 6 |
Journal | Burns |
Volume | 32 |
Issue number | 5 |
DOIs | |
State | Published - Aug 2006 |
Keywords
- Cell-mediated immunity
- Estrogen
- Injury
- Macrophages
- Proinflammatory cytokines
- T lymphocytes
ASJC Scopus subject areas
- Surgery
- Emergency Medicine
- Critical Care and Intensive Care Medicine