Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI

Deborah I Friedman; J. Kelly Johnson; Regina L. Chorsky; Edward G. Stopa

doi:10.1016/0006-8993(91)91246-W

Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI

Deborah I Friedman, J. Kelly Johnson, Regina L. Chorsky, Edward G. Stopa

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The carbocyanine dye, DiI, was used to demonstrate human retinohypothalamic tract (RHT) projections in 6 normal human postmortem brains. In 5 of 6 brains, labeling was seen extending from the site of implantation in the distal optic nerve to both the ipsilateral and contralateral suprachiasmatic nuclei. This study confirms the presence of RHT projections in humans, and demonstrates the usefulness of DiI for neuronal tracing in human postmortem tissue.

Original language	English (US)
Pages (from-to)	297-302
Number of pages	6
Journal	Brain Research
Volume	560
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(91)91246-W
State	Published - Sep 27 1991

Keywords

Carbocyanine dye
Circadian rhythm
DiI
Hypothalamus
Retinohypothalamic tract
Suprachiamatic nucleus

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(91)91246-W

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title = "Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI",

abstract = "The carbocyanine dye, DiI, was used to demonstrate human retinohypothalamic tract (RHT) projections in 6 normal human postmortem brains. In 5 of 6 brains, labeling was seen extending from the site of implantation in the distal optic nerve to both the ipsilateral and contralateral suprachiasmatic nuclei. This study confirms the presence of RHT projections in humans, and demonstrates the usefulness of DiI for neuronal tracing in human postmortem tissue.",

keywords = "Carbocyanine dye, Circadian rhythm, DiI, Hypothalamus, Retinohypothalamic tract, Suprachiamatic nucleus",

author = "Friedman, {Deborah I} and {Kelly Johnson}, J. and Chorsky, {Regina L.} and Stopa, {Edward G.}",

note = "Funding Information: Andrew Satlin, George Collins and Ellion Albers for many helpful suggestions, and Dr. Edward Bird for providing some of the tissues used in this study. Supported by A~95 and AG09301 (E.G.S.), Brain Bank Grant MI-I/NS31862, SUNY Health Science Center Pathology Medical Service Group (E.G.S.) and the Hen-dricks Fund for Medical Research (D.I.E, E.G.S.).",

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doi = "10.1016/0006-8993(91)91246-W",

language = "English (US)",

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AU - Kelly Johnson, J.

AU - Chorsky, Regina L.

AU - Stopa, Edward G.

N1 - Funding Information: Andrew Satlin, George Collins and Ellion Albers for many helpful suggestions, and Dr. Edward Bird for providing some of the tissues used in this study. Supported by A~95 and AG09301 (E.G.S.), Brain Bank Grant MI-I/NS31862, SUNY Health Science Center Pathology Medical Service Group (E.G.S.) and the Hen-dricks Fund for Medical Research (D.I.E, E.G.S.).

PY - 1991/9/27

Y1 - 1991/9/27

N2 - The carbocyanine dye, DiI, was used to demonstrate human retinohypothalamic tract (RHT) projections in 6 normal human postmortem brains. In 5 of 6 brains, labeling was seen extending from the site of implantation in the distal optic nerve to both the ipsilateral and contralateral suprachiasmatic nuclei. This study confirms the presence of RHT projections in humans, and demonstrates the usefulness of DiI for neuronal tracing in human postmortem tissue.

AB - The carbocyanine dye, DiI, was used to demonstrate human retinohypothalamic tract (RHT) projections in 6 normal human postmortem brains. In 5 of 6 brains, labeling was seen extending from the site of implantation in the distal optic nerve to both the ipsilateral and contralateral suprachiasmatic nuclei. This study confirms the presence of RHT projections in humans, and demonstrates the usefulness of DiI for neuronal tracing in human postmortem tissue.

KW - Carbocyanine dye

KW - Circadian rhythm

KW - DiI

KW - Hypothalamus

KW - Retinohypothalamic tract

KW - Suprachiamatic nucleus

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Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI

Abstract

Keywords

ASJC Scopus subject areas

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