Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI

Deborah I Friedman, J. Kelly Johnson, Regina L. Chorsky, Edward G. Stopa

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


The carbocyanine dye, DiI, was used to demonstrate human retinohypothalamic tract (RHT) projections in 6 normal human postmortem brains. In 5 of 6 brains, labeling was seen extending from the site of implantation in the distal optic nerve to both the ipsilateral and contralateral suprachiasmatic nuclei. This study confirms the presence of RHT projections in humans, and demonstrates the usefulness of DiI for neuronal tracing in human postmortem tissue.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalBrain Research
Issue number1-2
StatePublished - Sep 27 1991


  • Carbocyanine dye
  • Circadian rhythm
  • DiI
  • Hypothalamus
  • Retinohypothalamic tract
  • Suprachiamatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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