Abstract
Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.
Original language | English (US) |
---|---|
Pages (from-to) | 266-271 |
Number of pages | 6 |
Journal | Analytical biochemistry |
Volume | 193 |
Issue number | 2 |
DOIs | |
State | Published - Mar 1991 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology