Isolation and patch-clamp of primary adipocytes

Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, Isaac Samuel, Jessica K. Smith, Rajan Sah

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature adipocytes from both mouse and human white adipose tissues (subcutaneous and visceral). From the same preparation, we describe how to culture and differentiate preadipocytes isolated from the stromal vascular fraction. We then describe in detail patch-clamp methods, including both whole-cell and perforated-patch configurations.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages6
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Adipocyte isolation
  • Culture
  • Differentiation
  • Patch-clamp
  • Perforated-patch
  • Whole-cell patch-clamp

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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