TY - JOUR
T1 - Isolation and characterization of a glycine-extended form of calcitonin receptor-stimulating peptide-1
T2 - Another biologically active form of calcitonin receptor-stimulating peptide-1
AU - Katafuchi, Takeshi
AU - Hamano, Kazumasa
AU - Kikumoto, Katsuro
AU - Minamino, Naoto
N1 - Funding Information:
This work was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology (Special Coordination Funds for the Promotion of Science and Technology), from the Ministry of Health, Labor and Welfare (Cardiovascular Diseases), from the Pharmaceuticals and Medical Devices Agency (Medical Frontier Project) of Japan, and from the Protein Research Foundation (Kaneko-Narita Research Grant). The authors are grateful to Ms. A. Okabe, Y. Takada and S. Fujiwara of this institute for their technical assistance.
PY - 2005/12
Y1 - 2005/12
N2 - In this study, we isolated a peptide eliciting a potent stimulatory effect on cAMP production in LLC-PK1 cells from acid extracts of porcine brain. By structural analysis, this peptide was determined to be a C-terminal glycine-extended form of calcitonin receptor-stimulating peptide-1 (CRSP-1-Gly). Synthetic CRSP-1-Gly enhanced the cAMP production in COS-7 cells expressing calcitonin (CT) receptor as strongly as CRSP-1. Measurement of immunoreactive (IR) CRSP-1-Gly by radioimmunoassay using the specific antisera against CRSP-1-Gly showed that a relatively high level (>1 pmol/g wet weight) of IR-CRSP-1-Gly was detected in the midbrain, hypothalamus, anterior and posterior lobes of pituitary, and thyroid gland, and the ratio of IR-CRSP-1-Gly to total IR-CRSP-1 varies from 0.02 to 0.35 in each tissue. These results suggest that CRSP-1-Gly is actually present in the tissues as one of major endogenous molecular forms of CRSP-1, and can regulate the cells expressing the CT receptor both in the central nervous system and peripheral tissues in a manner similar to that of CRSP-1. IR-CRSP-2 and IR-CRSP-3 are also present in the brain and other tissues, but their tissue concentrations are 33% on average and less than 3% that of total IR-CRSP-1, respectively.
AB - In this study, we isolated a peptide eliciting a potent stimulatory effect on cAMP production in LLC-PK1 cells from acid extracts of porcine brain. By structural analysis, this peptide was determined to be a C-terminal glycine-extended form of calcitonin receptor-stimulating peptide-1 (CRSP-1-Gly). Synthetic CRSP-1-Gly enhanced the cAMP production in COS-7 cells expressing calcitonin (CT) receptor as strongly as CRSP-1. Measurement of immunoreactive (IR) CRSP-1-Gly by radioimmunoassay using the specific antisera against CRSP-1-Gly showed that a relatively high level (>1 pmol/g wet weight) of IR-CRSP-1-Gly was detected in the midbrain, hypothalamus, anterior and posterior lobes of pituitary, and thyroid gland, and the ratio of IR-CRSP-1-Gly to total IR-CRSP-1 varies from 0.02 to 0.35 in each tissue. These results suggest that CRSP-1-Gly is actually present in the tissues as one of major endogenous molecular forms of CRSP-1, and can regulate the cells expressing the CT receptor both in the central nervous system and peripheral tissues in a manner similar to that of CRSP-1. IR-CRSP-2 and IR-CRSP-3 are also present in the brain and other tissues, but their tissue concentrations are 33% on average and less than 3% that of total IR-CRSP-1, respectively.
KW - Calcitonin receptor
KW - Calcitonin receptor-stimulating peptide
KW - Glycine-extended form of calcitonin receptor-stimulating peptide
KW - Isolation
KW - Radioimmunoassay
KW - cAMP
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U2 - 10.1016/j.peptides.2005.06.004
DO - 10.1016/j.peptides.2005.06.004
M3 - Article
C2 - 16023259
AN - SCOPUS:27944475961
SN - 0196-9781
VL - 26
SP - 2616
EP - 2623
JO - Peptides
JF - Peptides
IS - 12
ER -