TY - JOUR
T1 - Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions
AU - Mineo, Chieko
AU - Murakami, Yasufumi
AU - Ishimi, Yukio
AU - Hanaoka, Fumio
AU - Yamada, Masa Atsu
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1986/11
Y1 - 1986/11
N2 - A temperature-sensitive (ts) mutant, designated tsFT210, was isolated from a mouse mammary carcinoma cell line, FM3A. The tsFT210 cells grew normally at 33 °C (permissive temperature), but more than 80% of the cells were arrested at the G2 phase at 39 °C (nonpermissive temperature) as revealed by flow-microfluorimetric analysis. DNA replication and synthesis of other macromolecules by this mutant seemed to be normal at 39 °C for at least 10 h. However, in this mutant, hyperphosphorylation of H1 histone from the G2 to M phase, which occurs in the normal cell cycle, could not be detected at the non-permissive temperature. This suggests that a gene product which is temperature-sensitive in tsFT210 cells is necessary for hyperphosphorylation of H1 histone and that this gene product may be related to chromosome condensation.
AB - A temperature-sensitive (ts) mutant, designated tsFT210, was isolated from a mouse mammary carcinoma cell line, FM3A. The tsFT210 cells grew normally at 33 °C (permissive temperature), but more than 80% of the cells were arrested at the G2 phase at 39 °C (nonpermissive temperature) as revealed by flow-microfluorimetric analysis. DNA replication and synthesis of other macromolecules by this mutant seemed to be normal at 39 °C for at least 10 h. However, in this mutant, hyperphosphorylation of H1 histone from the G2 to M phase, which occurs in the normal cell cycle, could not be detected at the non-permissive temperature. This suggests that a gene product which is temperature-sensitive in tsFT210 cells is necessary for hyperphosphorylation of H1 histone and that this gene product may be related to chromosome condensation.
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U2 - 10.1016/0014-4827(86)90203-X
DO - 10.1016/0014-4827(86)90203-X
M3 - Article
C2 - 2428648
AN - SCOPUS:0023010845
SN - 0014-4827
VL - 167
SP - 53
EP - 62
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -