Isolating lipid droplets from multiple species

Yunfeng Ding, Shuyan Zhang, Li Yang, Huimin Na, Peng Zhang, Huina Zhang, Yang Wang, Yong Chen, Jinhai Yu, Chaoxing Huo, Shimeng Xu, Martina Garaiova, Yusheng Cong, Pingsheng Liu

Research output: Contribution to journalArticlepeer-review

116 Scopus citations


The lipid droplet (LD) is a cell organelle that has been linked to human metabolic syndromes and that can be exploited for the development of biofuels. The isolation of LDs is crucial for carrying out morphological and biochemical studies of this organelle. In the past two decades, LDs have been isolated from several organisms and investigated by microscopy, proteomics and lipidomics. However, these studies need to be extended to more model organisms, as well as to more animal tissues. Thus, a standard method that can be easily applied to these new samples with the need for minimal optimization is essential. Here we provide an LD isolation protocol that is relatively simple and suitable for a wide range of tissues and organisms. On the basis of previous studies, this 7-h protocol can yield 15-100 μg of protein-equivalent high-quality LDs that satisfy the requirements for current LD research in most organisms.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalNature Protocols
Issue number1
StatePublished - Jan 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'Isolating lipid droplets from multiple species'. Together they form a unique fingerprint.

Cite this