IRF4 is a key thermogenic transcriptional partner of PGC-1α

Xingxing Kong, Alexander Banks, Tiemin Liu, Lawrence Kazak, Rajesh R. Rao, Paul Cohen, Xun Wang, Songtao Yu, James C. Lo, Yu Hua Tseng, Aaron M. Cypess, Ruidan Xue, Sandra Kleiner, Sona Kang, Bruce M. Spiegelman, Evan D. Rosen

Research output: Contribution to journalArticlepeer-review

202 Scopus citations


Brown fat can reduce obesity through the dissipation of calories as heat. Control of thermogenic gene expression occurs via the induction of various coactivators, most notably PGC-1α. In contrast, the transcription factor partner(s) of these cofactors are poorly described. Here, we identify interferon regulatory factor 4 (IRF4) as a dominant transcriptional effector of thermogenesis. IRF4 is induced by cold and cAMP in adipocytes and is sufficient to promote increased thermogenic gene expression, energy expenditure, and cold tolerance. Conversely, knockout of IRF4 in UCP1+ cells causes reduced thermogenic gene expression and energy expenditure, obesity, and cold intolerance. IRF4 also induces the expression of PGC-1α and PRDM16 and interacts with PGC-1α, driving Ucp1 expression. Finally, cold, β-agonists, or forced expression of PGC-1α are unable to cause thermogenic gene expression in the absence of IRF4. These studies establish IRF4 as a transcriptional driver of a program of thermogenic gene expression and energy expenditure.

Original languageEnglish (US)
Pages (from-to)69-83
Number of pages15
Issue number1
StatePublished - Jul 3 2014

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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