Inhibitory effects of α-cyano-4-hydroxycinnamic acid on the activity of mushroom tyrosinase

Ling Qiu; Qiong Hua Chen; Jiang Xing Zhuang; Xue Zhong; Jing Jing Zhou; Yun Ji Guo; Qing Xi Chen

doi:10.1016/j.foodchem.2008.06.021

Inhibitory effects of α-cyano-4-hydroxycinnamic acid on the activity of mushroom tyrosinase

Ling Qiu, Qiong Hua Chen, Jiang Xing Zhuang, Xue Zhong, Jing Jing Zhou, Yun Ji Guo, Qing Xi Chen

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

The effects of α-cyano-4-hydroxycinnamic acid (HCCA) on the activity of mushroom tyrosinase have been studied. Results showed that HCCA could inhibit both the monophenolase activity and diphenolase activity of mushroom tyrosinase. For the monophenolase activity, the lag phase was obviously lengthened, and the steady-state activity of the enzyme decreased sharply. When the concentration of HCCA reached to 80 μM, the lag time was lengthened from 20 s to 150 s and the steady-state activity was lost by about 75%. The IC₅₀ value was estimated to be 48 μM. For the diphenolase activity, the inhibitory effect of HCCA was also dose-dependent and the IC₅₀ value was estimated to be 2.17 mM. The kinetic analyses showed that the inhibition of HCCA on the diphenolase activity was reversible and competitive with the inhibition constants (K_I) determined to be 1.24 mM.

Original language	English (US)
Pages (from-to)	609-613
Number of pages	5
Journal	Food Chemistry
Volume	112
Issue number	3
DOIs	https://doi.org/10.1016/j.foodchem.2008.06.021
State	Published - Feb 1 2009
Externally published	Yes

Keywords

Diphenolase activity
Inhibition mechanism
Kinetics
Monophenolase activity
Mushroom tyrosinase
α-Cyano-4-hydroxycinnamic acid

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2008.06.021

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@article{3aa239bc596d4912842e0b1e22f0a2d9,

title = "Inhibitory effects of α-cyano-4-hydroxycinnamic acid on the activity of mushroom tyrosinase",

abstract = "The effects of α-cyano-4-hydroxycinnamic acid (HCCA) on the activity of mushroom tyrosinase have been studied. Results showed that HCCA could inhibit both the monophenolase activity and diphenolase activity of mushroom tyrosinase. For the monophenolase activity, the lag phase was obviously lengthened, and the steady-state activity of the enzyme decreased sharply. When the concentration of HCCA reached to 80 μM, the lag time was lengthened from 20 s to 150 s and the steady-state activity was lost by about 75%. The IC50 value was estimated to be 48 μM. For the diphenolase activity, the inhibitory effect of HCCA was also dose-dependent and the IC50 value was estimated to be 2.17 mM. The kinetic analyses showed that the inhibition of HCCA on the diphenolase activity was reversible and competitive with the inhibition constants (KI) determined to be 1.24 mM.",

keywords = "Diphenolase activity, Inhibition mechanism, Kinetics, Monophenolase activity, Mushroom tyrosinase, α-Cyano-4-hydroxycinnamic acid",

author = "Ling Qiu and Chen, {Qiong Hua} and Zhuang, {Jiang Xing} and Xue Zhong and Zhou, {Jing Jing} and Guo, {Yun Ji} and Chen, {Qing Xi}",

note = "Funding Information: The present investigation was supported by Program for Innovative Research Team in Science and Technology in Fujian Province University, and by Grant 30570408 of the Natural Science Foundation of China, Grant 2007N0051 of the Science and Technology Foundation of Fujian Province.",

year = "2009",

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doi = "10.1016/j.foodchem.2008.06.021",

language = "English (US)",

volume = "112",

pages = "609--613",

journal = "Food Chemistry",

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T1 - Inhibitory effects of α-cyano-4-hydroxycinnamic acid on the activity of mushroom tyrosinase

AU - Qiu, Ling

AU - Chen, Qiong Hua

AU - Zhuang, Jiang Xing

AU - Zhong, Xue

AU - Zhou, Jing Jing

AU - Guo, Yun Ji

AU - Chen, Qing Xi

N1 - Funding Information: The present investigation was supported by Program for Innovative Research Team in Science and Technology in Fujian Province University, and by Grant 30570408 of the Natural Science Foundation of China, Grant 2007N0051 of the Science and Technology Foundation of Fujian Province.

PY - 2009/2/1

Y1 - 2009/2/1

N2 - The effects of α-cyano-4-hydroxycinnamic acid (HCCA) on the activity of mushroom tyrosinase have been studied. Results showed that HCCA could inhibit both the monophenolase activity and diphenolase activity of mushroom tyrosinase. For the monophenolase activity, the lag phase was obviously lengthened, and the steady-state activity of the enzyme decreased sharply. When the concentration of HCCA reached to 80 μM, the lag time was lengthened from 20 s to 150 s and the steady-state activity was lost by about 75%. The IC50 value was estimated to be 48 μM. For the diphenolase activity, the inhibitory effect of HCCA was also dose-dependent and the IC50 value was estimated to be 2.17 mM. The kinetic analyses showed that the inhibition of HCCA on the diphenolase activity was reversible and competitive with the inhibition constants (KI) determined to be 1.24 mM.

AB - The effects of α-cyano-4-hydroxycinnamic acid (HCCA) on the activity of mushroom tyrosinase have been studied. Results showed that HCCA could inhibit both the monophenolase activity and diphenolase activity of mushroom tyrosinase. For the monophenolase activity, the lag phase was obviously lengthened, and the steady-state activity of the enzyme decreased sharply. When the concentration of HCCA reached to 80 μM, the lag time was lengthened from 20 s to 150 s and the steady-state activity was lost by about 75%. The IC50 value was estimated to be 48 μM. For the diphenolase activity, the inhibitory effect of HCCA was also dose-dependent and the IC50 value was estimated to be 2.17 mM. The kinetic analyses showed that the inhibition of HCCA on the diphenolase activity was reversible and competitive with the inhibition constants (KI) determined to be 1.24 mM.

KW - Diphenolase activity

KW - Inhibition mechanism

KW - Kinetics

KW - Monophenolase activity

KW - Mushroom tyrosinase

KW - α-Cyano-4-hydroxycinnamic acid

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Inhibitory effects of α-cyano-4-hydroxycinnamic acid on the activity of mushroom tyrosinase

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Keywords

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