Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes

Jeffrey McKinney; Cecilia Guerrier-Takada; Donna Wesolowski; Sidney Altman

doi:10.1073/pnas.121180398

Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes

Jeffrey McKinney, Cecilia Guerrier-Takada, Donna Wesolowski, Sidney Altman

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In both cases, external guide sequences (EGS) have been designed to complex with either mRNA. Using the EGS technology, the level of microbial viability is reduced to less than 10% of the wild-type strain. The EGSs are additive when used together and depend on the number of nucleotides paired when attacking gyrase A mRNA. In the case of gyrase A, three nucleotides unpaired out of a 15-mer EGS still favor complete inhibition by the EGS but five unpaired nucleotides do not.

Original language	English (US)
Pages (from-to)	6605-6610
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	12
DOIs	https://doi.org/10.1073/pnas.121180398
State	Published - Jun 5 2001

Keywords

Rnase P
tRNA processing

ASJC Scopus subject areas

General

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10.1073/pnas.121180398

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Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes. / McKinney, Jeffrey; Guerrier-Takada, Cecilia; Wesolowski, Donna et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 12, 05.06.2001, p. 6605-6610.

Research output: Contribution to journal › Article › peer-review

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Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes

Abstract

Keywords

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