TY - JOUR
T1 - Inflammatory markers for improved recurrent UTI diagnosis in postmenopausal women
AU - Ebrahimzadeh, Tahmineh
AU - Basu, Ujjaini
AU - Lutz, Kevin C.
AU - Gadhvi, Jashkaran
AU - Komarovsky, Jessica V.
AU - Li, Qiwei
AU - Zimmern, Philippe E.
AU - De Nisco, Nicole J.
N1 - Publisher Copyright:
© 2024 Ebrahimzadeh et al.
PY - 2024/4
Y1 - 2024/4
N2 - Recurrent urinary tract infection (rUTI) severely impacts post-menopausal women. The lack of rapid and accurate diagnostic tools is a major obstacle in rUTI management as current gold standard methods have >24-h diagnostic windows. Work in ani-mal models and limited human cohorts have identified robust inflammatory responses activated during UTI. Consequently, urinary inflammatory cytokines secreted during UTI may function as diagnostic biomarkers. This study aimed to identify urinary cytokines that could accurately diagnose UTI in a controlled cohort of postmenopausal women. Women passing study ex-clusion criteria were classified into no UTI and active rUTI groups, and urinary cytokine levels were measured by immunoassay. Pro-inflammatory cytokines IL-8, IL-18, IL-1β, and monocyte chemo-attractant protein-1 were significantly elevated in the active rUTI group, and anti-inflammatory cytokines IL-13 and IL-4 were elevated in women without UTI. We evaluated cytokine diagnostic performance and found that an IL-8, prostaglandin E2, and IL-13 multivariable model had the lowest misclassification rate and highest sensitivity. Our data identify urinary IL-8, prostaglandin E2, and IL-13 as candidate biomarkers that may be useful in the development of immunoassay-based UTI diagnostics.
AB - Recurrent urinary tract infection (rUTI) severely impacts post-menopausal women. The lack of rapid and accurate diagnostic tools is a major obstacle in rUTI management as current gold standard methods have >24-h diagnostic windows. Work in ani-mal models and limited human cohorts have identified robust inflammatory responses activated during UTI. Consequently, urinary inflammatory cytokines secreted during UTI may function as diagnostic biomarkers. This study aimed to identify urinary cytokines that could accurately diagnose UTI in a controlled cohort of postmenopausal women. Women passing study ex-clusion criteria were classified into no UTI and active rUTI groups, and urinary cytokine levels were measured by immunoassay. Pro-inflammatory cytokines IL-8, IL-18, IL-1β, and monocyte chemo-attractant protein-1 were significantly elevated in the active rUTI group, and anti-inflammatory cytokines IL-13 and IL-4 were elevated in women without UTI. We evaluated cytokine diagnostic performance and found that an IL-8, prostaglandin E2, and IL-13 multivariable model had the lowest misclassification rate and highest sensitivity. Our data identify urinary IL-8, prostaglandin E2, and IL-13 as candidate biomarkers that may be useful in the development of immunoassay-based UTI diagnostics.
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U2 - 10.26508/lsa.202302323
DO - 10.26508/lsa.202302323
M3 - Article
C2 - 38331474
AN - SCOPUS:85184695376
SN - 2575-1077
VL - 7
JO - Life Science Alliance
JF - Life Science Alliance
IS - 4
M1 - e202302323
ER -