Industrial and Ruminant Trans-Fatty Acids-Enriched Diets Differentially Modulate the Microbiome and Fecal Metabolites in C57BL/6 Mice

Farzad Mohammadi, Miranda Green, Emma Tolsdorf, Karine Greffard, Mickael Leclercq, Jean François Bilodeau, Arnaud Droit, Jane Foster, Nicolas Bertrand, Iwona Rudkowska

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Industrially originated trans-fatty acids (I-tFAs), such as elaidic acid (EA), and ruminant trans-fatty acids (R-tFAs), such as trans-palmitoleic acid (TPA), may have opposite effects on metabolic health. The objective was to compare the effects of consuming 2–3% I-tFA or R-tFA on the gut microbiome and fecal metabolite profile in mice after 7 and 28 days. Forty C57BL/6 mice were assigned to one of the four prepared formulations: lecithin nanovesicles, lecithin nanovesicles with EA or TPA, or water. Fecal samples and animals’ weights were collected on days 0, 7, and 28. Fecal samples were used to determine gut microbiome profiles by 16S rRNA sequencing and metabolite concentrations by GC/MS. At 28 days, TPA intake decreased the abundance of Staphylococcus sp55 but increased Staphylococcus sp119. EA intake also increased the abundance of Staphylococcus sp119 but decreased Ruminococcaceae UCG-014, Lachnospiraceae, and Clostridium sensu stricto 1 at 28 days. Fecal short-chain fatty acids were increased after TPA while decreased after EA after 7 and 28 days. This study shows that TPA and EA modify the abundance of specific microbial taxa and fecal metabolite profiles in distinct ways.

Original languageEnglish (US)
Article number1433
JournalNutrients
Volume15
Issue number6
DOIs
StatePublished - Mar 2023
Externally publishedYes

Keywords

  • industrial trans-fatty acid (I-tFA)
  • metabolites
  • microbiomes
  • ruminant trans-fatty acid (R-tFA)
  • short-chain fatty acids (SCFAs)

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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