TY - JOUR
T1 - Incorporaton of radioactive phospholipid into subclasses of high-density lipoproteins
AU - Tall, A. R.
AU - Blum, C. B.
AU - Grundy, Scott M
PY - 1983
Y1 - 1983
N2 - The incorporation of orally administered phospholipid into plasma high-density lipoproteins (HDL) was studied in three subjects. Plasma was analyzed by equilibrium density gradient ultracentrifugation, 5, 6, and 8 h after ingestion of 1.1 g [3H-choline, 14C-dilinoleoyl]phosphatidylcholine. At all time points in all subjects, there was a peak of phosphatidylcholine specific activity in fractions of density approximately 1.10-1.13 g/ml, corresponding to the subclass previously designated HDL(2a). There was also a more variable, smaller peak of specific activity of phospholipids in HDL(2b) (1.063-1.100 g/ml) and in fractions of density ~ 1.19 g/ml. In the 1.10-1.13 fraction, 97 and 71%, respectively, of the 3H and 14C radioactivity were in phospholipids. The 3H/14C rato was similar in phospholipids of HDL subfractions, the d < 1.07 fraction, and in the administered phospholipid. The results show preferential transfer or exchange or absorbed phosphatidylcholine into specific subclasses of HDL.
AB - The incorporation of orally administered phospholipid into plasma high-density lipoproteins (HDL) was studied in three subjects. Plasma was analyzed by equilibrium density gradient ultracentrifugation, 5, 6, and 8 h after ingestion of 1.1 g [3H-choline, 14C-dilinoleoyl]phosphatidylcholine. At all time points in all subjects, there was a peak of phosphatidylcholine specific activity in fractions of density approximately 1.10-1.13 g/ml, corresponding to the subclass previously designated HDL(2a). There was also a more variable, smaller peak of specific activity of phospholipids in HDL(2b) (1.063-1.100 g/ml) and in fractions of density ~ 1.19 g/ml. In the 1.10-1.13 fraction, 97 and 71%, respectively, of the 3H and 14C radioactivity were in phospholipids. The 3H/14C rato was similar in phospholipids of HDL subfractions, the d < 1.07 fraction, and in the administered phospholipid. The results show preferential transfer or exchange or absorbed phosphatidylcholine into specific subclasses of HDL.
UR - http://www.scopus.com/inward/record.url?scp=17744413680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17744413680&partnerID=8YFLogxK
M3 - Article
C2 - 6846536
AN - SCOPUS:17744413680
SN - 0193-1857
VL - 7
SP - E513-E516
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 5
ER -