In vivo imaging of small animals with optical tomography and near infrared fluorescent probes

Matthew R. Palmer, Yasushi Shibata, Jonathan B. Kruskal, Robert E. Lenkinski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations


A developmental optical tomograph has been designed for imaging small animals in vivo using near infrared (NIR) fluorphores. The system employs epi-illumnation via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high-resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180° rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions from ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.J. Bornhop, D.A. Dunn, R.P. Mariella, Jr., C.J. Murphy, others and others
Number of pages4
StatePublished - 2002
EventBiomedical Nanotechnology Architectures and Applications - San Jose, CA, United States
Duration: Jan 20 2002Jan 24 2002


OtherBiomedical Nanotechnology Architectures and Applications
Country/TerritoryUnited States
CitySan Jose, CA


  • Fluorescence
  • Indocyanine green
  • Optical imaging

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics


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