In vivo dynamics of T cells and their interactions with dendritic cells in mouse cutaneous graft-versus-host disease

Graft-versus-host disease (GVHD) is a major cause of morbidity and mortality in allogeneic hematopoietic stem cell transplantation (alloSCT). By static microscopy, cutaneous GVHD lesions contain a mix of T cells and myeloid cells. We used 2-photon intravital microscopy to investigate the dynamics of CD4¹ and CD8¹ T cells and donor dendritic cells (DCs) in cutaneous GVHD lesions in an MHC-matched, multiple minor histocompatibility antigen-mismatched (miHA) model. The majority of CD4 and CD8 cells were stationary, and few cells entered and stopped or were stopped and left the imaged volumes. CD8 cells made TCR:MHCI-dependent interactions with CD11c¹ cells, as measured by the durations that CD8 cells contacted MHCI¹ vs MHCI² DCs. The acute deletion of Langerin¹CD103¹ DCs, which were relatively rare, did not affect CD8 cell motility and DC contact times, indicating that Langerin²CD103² DCs provide stop signals to CD8 cells. CD4 cells, in contrast, had similar contact durations with MHCII¹ and MHCII² DCs. However, CD4 motility rapidly increased after the infusion of an MHCII-blocking antibody, indicating that TCR signaling actively suppressed CD4 movements. Many CD4 cells still were stationary after anti-MHCII antibody infusion, suggesting CD4 cell heterogeneity within the lesion. These data support a model of local GVHD maintenance within target tissues.