In vivo delivery of pPERDBY to BALB/c mice by LacVax ® DNA-I and comparison of elicited immune response with conventional immunization methods

Bhrugu Yagnik; Drashya Sharma; Harish Padh; Priti Desai

doi:10.1038/s41434-018-0033-8

In vivo delivery of pPERDBY to BALB/c mice by LacVax ^® DNA-I and comparison of elicited immune response with conventional immunization methods

Bhrugu Yagnik, Drashya Sharma, Harish Padh, Priti Desai

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The non-invasive food grade Lactococcus lactis (L. lactis) represents a safe and attractive alternative to invasive pathogens for the delivery of plasmid DNA at mucosal sites. We have earlier shown the DNA delivery potential of r-L. lactis harboring DNA vaccine reporter plasmid; pPERDBY in vitro. In the present work, we examined in vivo delivery potential of food grade non-invasive r-L. lactis::pPERDBY (LacVax^®DNA-I) in BALB/c mice. Moreover, using EGFP as a model antigen, we also characterized and compared the immune response elicited by LacVax^®DNA-I with other conventional vaccination approaches using protein and naked DNA immunization. The presence of antigen-specific serum IgG and fecal secretory IgA (sIgA) antibodies demonstrated in vivo DNA delivery and immune elicitation potential of the developed LacVax^®DNA-I. As compared with intramuscular injection, oral delivery of pPERDBY via L. lactis resulted in a significantly rapid increase in IgG and higher sIgA titers, indicating the immunogenic and immunostimulatory properties of the LacVax^®DNA-I. The needle-free immunization with LacVax^®DNA-I led to increased production of IL-4, an indicator of Th2 screwed response. To the best of our knowledge, this report for the first time outlines comparison of orally administered LacVax^®DNA-I with other conventional vaccination approaches.

Original language	English (US)
Pages (from-to)	485-496
Number of pages	12
Journal	Gene Therapy
Volume	25
Issue number	7
DOIs	https://doi.org/10.1038/s41434-018-0033-8
State	Published - Oct 1 2018
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics

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title = "In vivo delivery of pPERDBY to BALB/c mice by LacVax {\textregistered} DNA-I and comparison of elicited immune response with conventional immunization methods",

abstract = "The non-invasive food grade Lactococcus lactis (L. lactis) represents a safe and attractive alternative to invasive pathogens for the delivery of plasmid DNA at mucosal sites. We have earlier shown the DNA delivery potential of r-L. lactis harboring DNA vaccine reporter plasmid; pPERDBY in vitro. In the present work, we examined in vivo delivery potential of food grade non-invasive r-L. lactis::pPERDBY (LacVax{\textregistered}DNA-I) in BALB/c mice. Moreover, using EGFP as a model antigen, we also characterized and compared the immune response elicited by LacVax{\textregistered}DNA-I with other conventional vaccination approaches using protein and naked DNA immunization. The presence of antigen-specific serum IgG and fecal secretory IgA (sIgA) antibodies demonstrated in vivo DNA delivery and immune elicitation potential of the developed LacVax{\textregistered}DNA-I. As compared with intramuscular injection, oral delivery of pPERDBY via L. lactis resulted in a significantly rapid increase in IgG and higher sIgA titers, indicating the immunogenic and immunostimulatory properties of the LacVax{\textregistered}DNA-I. The needle-free immunization with LacVax{\textregistered}DNA-I led to increased production of IL-4, an indicator of Th2 screwed response. To the best of our knowledge, this report for the first time outlines comparison of orally administered LacVax{\textregistered}DNA-I with other conventional vaccination approaches.",

author = "Bhrugu Yagnik and Drashya Sharma and Harish Padh and Priti Desai",

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AU - Desai, Priti

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In vivo delivery of pPERDBY to BALB/c mice by LacVax ^® DNA-I and comparison of elicited immune response with conventional immunization methods

Abstract

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