In-vitro GLP-1 Release Assay Using STC-1 Cells

Liu Qi; Tan Shuai; Jia Da; Burstein Ezra; Sifuentes Dominguez Luis

doi:10.21769/BioProtoc.3717

In-vitro GLP-1 Release Assay Using STC-1 Cells

Liu Qi, Tan Shuai, Jia Da, Burstein Ezra, Sifuentes Dominguez Luis

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.

Original language	English (US)
Article number	exxxx
Journal	Bio-protocol
Volume	10
Issue number	16
DOIs	https://doi.org/10.21769/BioProtoc.3717
State	Published - Aug 20 2020
Externally published	Yes

Keywords

Docosahexaenoic acid (DHA)
Enteroendocrine cells
Glucagon-like peptide-1 (GLP-1)
L-cells
STC-1

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
Plant Science

Access to Document

10.21769/BioProtoc.3717

Cite this

@article{cdee4d68a03a472c916974cb3e5f3c38,

title = "In-vitro GLP-1 Release Assay Using STC-1 Cells",

abstract = "Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC{\textquoteright}s under fatty acid stimulation.",

keywords = "Docosahexaenoic acid (DHA), Enteroendocrine cells, Glucagon-like peptide-1 (GLP-1), L-cells, STC-1",

author = "Liu Qi and Tan Shuai and Jia Da and Burstein Ezra and Luis, {Sifuentes Dominguez}",

note = "Publisher Copyright: Copyright Liu et al.",

year = "2020",

month = aug,

day = "20",

doi = "10.21769/BioProtoc.3717",

language = "English (US)",

volume = "10",

journal = "Bio-protocol",

issn = "2331-8325",

publisher = "Bio-protocol LLC",

number = "16",

}

TY - JOUR

T1 - In-vitro GLP-1 Release Assay Using STC-1 Cells

AU - Qi, Liu

AU - Shuai, Tan

AU - Da, Jia

AU - Ezra, Burstein

AU - Luis, Sifuentes Dominguez

N1 - Publisher Copyright: Copyright Liu et al.

PY - 2020/8/20

Y1 - 2020/8/20

N2 - Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.

AB - Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.

KW - Docosahexaenoic acid (DHA)

KW - Enteroendocrine cells

KW - Glucagon-like peptide-1 (GLP-1)

KW - L-cells

KW - STC-1

UR - http://www.scopus.com/inward/record.url?scp=85150686313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85150686313&partnerID=8YFLogxK

U2 - 10.21769/BioProtoc.3717

DO - 10.21769/BioProtoc.3717

M3 - Article

C2 - 33659381

AN - SCOPUS:85150686313

SN - 2331-8325

VL - 10

JO - Bio-protocol

JF - Bio-protocol

IS - 16

M1 - exxxx

ER -

In-vitro GLP-1 Release Assay Using STC-1 Cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this