In-vitro GLP-1 Release Assay Using STC-1 Cells

Liu Qi; Tan Shuai; Jia Da; Burstein Ezra; Sifuentes Dominguez Luis

doi:10.21769/BioProtoc.3717

In-vitro GLP-1 Release Assay Using STC-1 Cells

Liu Qi, Tan Shuai, Jia Da, Burstein Ezra, Sifuentes Dominguez Luis

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.

Original language	English (US)
Article number	exxxx
Journal	Bio-protocol
Volume	10
Issue number	16
DOIs	https://doi.org/10.21769/BioProtoc.3717
State	Published - Aug 20 2020
Externally published	Yes

Keywords

Docosahexaenoic acid (DHA)
Enteroendocrine cells
Glucagon-like peptide-1 (GLP-1)
L-cells
STC-1

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
Plant Science

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10.21769/BioProtoc.3717

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title = "In-vitro GLP-1 Release Assay Using STC-1 Cells",

abstract = "Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC{\textquoteright}s under fatty acid stimulation.",

keywords = "Docosahexaenoic acid (DHA), Enteroendocrine cells, Glucagon-like peptide-1 (GLP-1), L-cells, STC-1",

author = "Liu Qi and Tan Shuai and Jia Da and Burstein Ezra and Luis, {Sifuentes Dominguez}",

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doi = "10.21769/BioProtoc.3717",

language = "English (US)",

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AU - Qi, Liu

AU - Shuai, Tan

AU - Da, Jia

AU - Ezra, Burstein

AU - Luis, Sifuentes Dominguez

N1 - Publisher Copyright: Copyright Liu et al.

PY - 2020/8/20

Y1 - 2020/8/20

N2 - Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.

AB - Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.

KW - Docosahexaenoic acid (DHA)

KW - Enteroendocrine cells

KW - Glucagon-like peptide-1 (GLP-1)

KW - L-cells

KW - STC-1

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JO - Bio-protocol

JF - Bio-protocol

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M1 - exxxx

ER -

In-vitro GLP-1 Release Assay Using STC-1 Cells

Abstract

Keywords

ASJC Scopus subject areas

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