TY - JOUR
T1 - In vitro evaluation of lipids adsorbed on silicone hydrogel contact lenses using a new gas chromatography/mass spectrometry analytical method.
AU - Iwata, Miyoko
AU - Ohno, Sadanori
AU - Kawai, Tetsuji
AU - Ichijima, Hideji
AU - Cavanagh, H. Dwight
PY - 2008
Y1 - 2008
N2 - PURPOSE: To establish a new analytical method using gas chromatography/mass spectrometry (GC/MS) for evaluating the lipids adsorbed on silicone hydrogel (SH) contact lenses: cholesterol, cholesterol ester, wax, and squalene. METHODS: A novel GC/MS method was developed and validated for the repeatability, specificity, linearity, detection and quantitation limits, and percentage recovery. The lipids in an artificial tear solution were adsorbed on 5 SH lenses (asmofilcon A, balafilcon A, galyfilcon A, lotrafilcon A, and lotrafilcon B) and 1 conventional hydrogel lens (etafilcon A) in vitro. The lipids adsorbed were then extracted and analyzed by the GC/MS method. RESULTS: Repeatability of this analytical method was less than 2.2% deviation for all test lipids; however, the analytes were completely discriminated with sharp peak shapes, and identified specifically. The correlation coefficient showing linearity was at least 0.991 under 50 microg/mL of lipid concentration. Detection and quantitation limits were statistically 0.5 to 0.8 microg/mL and 1.4 to 2.5 microg/mL, respectively, for all analytes. Percentage recovery was estimated as approximately 80% for 3 microg/lens, 90% for 5 microg/lens, and almost 100% for larger amounts of lipids. Quantitatively, the lipids absorbed on contact lenses were lotrafilcon A = (near equal) lotrafilcon B = (near equal) etafilcon A < asmofilcon A < galyfilcon A = (near equal) balafilcon A with the value of 0.4 to 7.6 microg/lens. Lipid adsorption on SH lenses varied depending on the lipid components and lens surface properties. CONCLUSIONS: The GC/MS method established in this study is excellent for the repeatability, specificity, linearity, detection and quantitation limits, and percentage recovery, and provides a novel highly sensitive and useful tool for evaluating lipids adsorbed on SH lenses.
AB - PURPOSE: To establish a new analytical method using gas chromatography/mass spectrometry (GC/MS) for evaluating the lipids adsorbed on silicone hydrogel (SH) contact lenses: cholesterol, cholesterol ester, wax, and squalene. METHODS: A novel GC/MS method was developed and validated for the repeatability, specificity, linearity, detection and quantitation limits, and percentage recovery. The lipids in an artificial tear solution were adsorbed on 5 SH lenses (asmofilcon A, balafilcon A, galyfilcon A, lotrafilcon A, and lotrafilcon B) and 1 conventional hydrogel lens (etafilcon A) in vitro. The lipids adsorbed were then extracted and analyzed by the GC/MS method. RESULTS: Repeatability of this analytical method was less than 2.2% deviation for all test lipids; however, the analytes were completely discriminated with sharp peak shapes, and identified specifically. The correlation coefficient showing linearity was at least 0.991 under 50 microg/mL of lipid concentration. Detection and quantitation limits were statistically 0.5 to 0.8 microg/mL and 1.4 to 2.5 microg/mL, respectively, for all analytes. Percentage recovery was estimated as approximately 80% for 3 microg/lens, 90% for 5 microg/lens, and almost 100% for larger amounts of lipids. Quantitatively, the lipids absorbed on contact lenses were lotrafilcon A = (near equal) lotrafilcon B = (near equal) etafilcon A < asmofilcon A < galyfilcon A = (near equal) balafilcon A with the value of 0.4 to 7.6 microg/lens. Lipid adsorption on SH lenses varied depending on the lipid components and lens surface properties. CONCLUSIONS: The GC/MS method established in this study is excellent for the repeatability, specificity, linearity, detection and quantitation limits, and percentage recovery, and provides a novel highly sensitive and useful tool for evaluating lipids adsorbed on SH lenses.
UR - http://www.scopus.com/inward/record.url?scp=58149346029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149346029&partnerID=8YFLogxK
U2 - 10.1097/icl.0b013e318182f357
DO - 10.1097/icl.0b013e318182f357
M3 - Article
C2 - 18779667
AN - SCOPUS:58149346029
SN - 1542-2321
VL - 34
SP - 272
EP - 280
JO - Eye & contact lens
JF - Eye & contact lens
IS - 5
ER -