TY - JOUR
T1 - Impaired liver regeneration in the analbuminemic rat
AU - Hicks, Barry A.
AU - Drougas, James
AU - Arnaout, Walid
AU - Felcher, Andrew
AU - Moscioni, Albert D.
AU - Levenson, Stanley M.
AU - Demetriou, Achilles A.
N1 - Funding Information:
Supported by National Institutes of Health (NIDDK) DK38763.
PY - 1989/5
Y1 - 1989/5
N2 - Albumin has been implicated as both an inhibitor and a stimulator of liver regeneration. We examined the rate of liver regeneration following partial hepatectomy in rats which are genetically analbuminemic. Adult male analbuminemic and Sprague-Dawley control rats underwent either a 70% partial hepatectomy or a sham operation. Twenty-four hours postoperatively, rats were injected iv with tritiated thymidine and the liver remnants were resected 1 hr later. Liver weight, protein and DNA contents, and the rate of tritiated thymidine incorporation into DNA were determined. The rate of DNA synthesis in control sham-operated rats was greater (P < 0.05) than that of an albuminemic rats. Following partial hepatectomy, there was a statistically significant (P < 0.01) increase in DNA synthesis in control rats; no significant increase was noted in the analbuminemic rats. No significant differences in liver remnant weight or protein and DNA contents were noted between control and analbuminemic sham-operated or partially hepatectomized rats. Our data suggest that albumin plays a role in the regulation of DNA synthesis in both the resting and the regenerating liver and it may be essential in maintaining a normal rate of liver regeneration.
AB - Albumin has been implicated as both an inhibitor and a stimulator of liver regeneration. We examined the rate of liver regeneration following partial hepatectomy in rats which are genetically analbuminemic. Adult male analbuminemic and Sprague-Dawley control rats underwent either a 70% partial hepatectomy or a sham operation. Twenty-four hours postoperatively, rats were injected iv with tritiated thymidine and the liver remnants were resected 1 hr later. Liver weight, protein and DNA contents, and the rate of tritiated thymidine incorporation into DNA were determined. The rate of DNA synthesis in control sham-operated rats was greater (P < 0.05) than that of an albuminemic rats. Following partial hepatectomy, there was a statistically significant (P < 0.01) increase in DNA synthesis in control rats; no significant increase was noted in the analbuminemic rats. No significant differences in liver remnant weight or protein and DNA contents were noted between control and analbuminemic sham-operated or partially hepatectomized rats. Our data suggest that albumin plays a role in the regulation of DNA synthesis in both the resting and the regenerating liver and it may be essential in maintaining a normal rate of liver regeneration.
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U2 - 10.1016/0022-4804(89)90155-8
DO - 10.1016/0022-4804(89)90155-8
M3 - Article
C2 - 2716303
AN - SCOPUS:0024411429
SN - 0022-4804
VL - 46
SP - 427
EP - 430
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 5
ER -