Abstract
MOPC41 immunoglobulin κ gene constructs have been stably introduced into the mouse germ line to investigate the effects of deleting the conserved intronic sequences on gene expression. Intact κ genes containing 1.5 kilobase pairs of upstream and 8.5 kilobase pairs of downstream flanking sequences were highly expressed tissue-specifically, raising the total level of κ mRNA in spleens severalfold in most transgenic animals. This high expression was often accompanied by marked suppression of endogenous κ gene activity. Transgenes containing a deletion of the matrix association region (MAR) or both the MAR and enhancer were expressed tissue-specifically at mean levels only 2- and 3-fold lower, respectively, than that of intact transgenes. Therefore, while the intronic enhancer and MAR appear to play a quantitative role in gene expression, these sequences are not absolutely essential for transcriptional activation of rearranged κ genes in a normal developmental environment.
Original language | English (US) |
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Pages (from-to) | 21190-21195 |
Number of pages | 6 |
Journal | Journal of Biological Chemistry |
Volume | 264 |
Issue number | 35 |
State | Published - 1989 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology