Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies

Sandra Sciascia; Kristina Robson; Liangjin Zhu; Michael Garland; Shannon Grabosch; Joseph Kelamis; Will Messamore; Todd Bradley; Allison Sourk; Lindsey Westberg; Waco Goodnight; Krystina Tongson; Naomi Holloway; Zabi Wardak; Michelle Sudyka; Shriti Masrani; Sanket Chintalapati; Manuel Cagaanan; J. C. Brown John; Dean A. Stetler

doi:10.1080/08916930601185550

Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies

Sandra Sciascia, Kristina Robson, Liangjin Zhu, Michael Garland, Shannon Grabosch, Joseph Kelamis, Will Messamore, Todd Bradley, Allison Sourk, Lindsey Westberg, Waco Goodnight, Krystina Tongson, Naomi Holloway, Zabi Wardak, Michelle Sudyka, Shriti Masrani, Sanket Chintalapati, Manuel Cagaanan, J. C. Brown John, Dean A. Stetler

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Antibodies against the N-terminal (NT) but not the basic domain (BD), DNA binding regions of the largest subunit (S1) of RNA polymerase I (RNAPI) were detected in the sera of MRL-lpr/lpr lupus mice. Antibodies against both RNAPI(S1)-NT and -BD, as well as other systemic lupus erythematosus (SLE) autoantigens (La, ribosomal P proteins and Sm/RNP) were produced by rabbits immunized with anti-DNA antibodies that had been affinity purified from SLE patients. Immunization of nonautoimmune mice (Balb/c) with RNAPI(S1)-NT, RNAPI(S1)-BD, or La in the form of GST fusion proteins, induced production of anti-double-stranded (ds) DNA and anti-Sm/RNP. GST-P1 did not induce an anti-dsDNA response in these mice. These results demonstrate that RNAPI(S1)-NT, RNAPI(S1)-BD and La can participate in an anti-autoantigen/anti-DNA antibody loop during an SLE-like autoimmune response.

Original language	English (US)
Pages (from-to)	38-47
Number of pages	10
Journal	Autoimmunity
Volume	40
Issue number	1
DOIs	https://doi.org/10.1080/08916930601185550
State	Published - Feb 2007
Externally published	Yes

Keywords

Anti-70.kDa U1-RNP antibodies
Anti-dsDNA antibodies
Lupus
RNA polymerase I
SLE

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1080/08916930601185550

Cite this

Sciascia, S., Robson, K., Zhu, L., Garland, M., Grabosch, S., Kelamis, J., Messamore, W., Bradley, T., Sourk, A., Westberg, L., Goodnight, W., Tongson, K., Holloway, N., Wardak, Z., Sudyka, M., Masrani, S., Chintalapati, S., Cagaanan, M., Brown John, J. C., & Stetler, D. A. (2007). Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies. Autoimmunity, 40(1), 38-47. https://doi.org/10.1080/08916930601185550

Sciascia, S, Robson, K, Zhu, L, Garland, M, Grabosch, S, Kelamis, J, Messamore, W, Bradley, T, Sourk, A, Westberg, L, Goodnight, W, Tongson, K, Holloway, N, Wardak, Z, Sudyka, M, Masrani, S, Chintalapati, S, Cagaanan, M, Brown John, JC & Stetler, DA 2007, 'Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies', Autoimmunity, vol. 40, no. 1, pp. 38-47. https://doi.org/10.1080/08916930601185550

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title = "Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies",

abstract = "Antibodies against the N-terminal (NT) but not the basic domain (BD), DNA binding regions of the largest subunit (S1) of RNA polymerase I (RNAPI) were detected in the sera of MRL-lpr/lpr lupus mice. Antibodies against both RNAPI(S1)-NT and -BD, as well as other systemic lupus erythematosus (SLE) autoantigens (La, ribosomal P proteins and Sm/RNP) were produced by rabbits immunized with anti-DNA antibodies that had been affinity purified from SLE patients. Immunization of nonautoimmune mice (Balb/c) with RNAPI(S1)-NT, RNAPI(S1)-BD, or La in the form of GST fusion proteins, induced production of anti-double-stranded (ds) DNA and anti-Sm/RNP. GST-P1 did not induce an anti-dsDNA response in these mice. These results demonstrate that RNAPI(S1)-NT, RNAPI(S1)-BD and La can participate in an anti-autoantigen/anti-DNA antibody loop during an SLE-like autoimmune response.",

keywords = "Anti-70.kDa U1-RNP antibodies, Anti-dsDNA antibodies, Lupus, RNA polymerase I, SLE",

author = "Sandra Sciascia and Kristina Robson and Liangjin Zhu and Michael Garland and Shannon Grabosch and Joseph Kelamis and Will Messamore and Todd Bradley and Allison Sourk and Lindsey Westberg and Waco Goodnight and Krystina Tongson and Naomi Holloway and Zabi Wardak and Michelle Sudyka and Shriti Masrani and Sanket Chintalapati and Manuel Cagaanan and {Brown John}, {J. C.} and Stetler, {Dean A.}",

note = "Funding Information: This work was funded by the Francis V. Stevens Memorial fund (KUEA).",

year = "2007",

month = feb,

doi = "10.1080/08916930601185550",

language = "English (US)",

volume = "40",

pages = "38--47",

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T1 - Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies

AU - Sciascia, Sandra

AU - Robson, Kristina

AU - Zhu, Liangjin

AU - Garland, Michael

AU - Grabosch, Shannon

AU - Kelamis, Joseph

AU - Messamore, Will

AU - Bradley, Todd

AU - Sourk, Allison

AU - Westberg, Lindsey

AU - Goodnight, Waco

AU - Tongson, Krystina

AU - Holloway, Naomi

AU - Wardak, Zabi

AU - Sudyka, Michelle

AU - Masrani, Shriti

AU - Chintalapati, Sanket

AU - Cagaanan, Manuel

AU - Brown John, J. C.

AU - Stetler, Dean A.

N1 - Funding Information: This work was funded by the Francis V. Stevens Memorial fund (KUEA).

PY - 2007/2

Y1 - 2007/2

N2 - Antibodies against the N-terminal (NT) but not the basic domain (BD), DNA binding regions of the largest subunit (S1) of RNA polymerase I (RNAPI) were detected in the sera of MRL-lpr/lpr lupus mice. Antibodies against both RNAPI(S1)-NT and -BD, as well as other systemic lupus erythematosus (SLE) autoantigens (La, ribosomal P proteins and Sm/RNP) were produced by rabbits immunized with anti-DNA antibodies that had been affinity purified from SLE patients. Immunization of nonautoimmune mice (Balb/c) with RNAPI(S1)-NT, RNAPI(S1)-BD, or La in the form of GST fusion proteins, induced production of anti-double-stranded (ds) DNA and anti-Sm/RNP. GST-P1 did not induce an anti-dsDNA response in these mice. These results demonstrate that RNAPI(S1)-NT, RNAPI(S1)-BD and La can participate in an anti-autoantigen/anti-DNA antibody loop during an SLE-like autoimmune response.

AB - Antibodies against the N-terminal (NT) but not the basic domain (BD), DNA binding regions of the largest subunit (S1) of RNA polymerase I (RNAPI) were detected in the sera of MRL-lpr/lpr lupus mice. Antibodies against both RNAPI(S1)-NT and -BD, as well as other systemic lupus erythematosus (SLE) autoantigens (La, ribosomal P proteins and Sm/RNP) were produced by rabbits immunized with anti-DNA antibodies that had been affinity purified from SLE patients. Immunization of nonautoimmune mice (Balb/c) with RNAPI(S1)-NT, RNAPI(S1)-BD, or La in the form of GST fusion proteins, induced production of anti-double-stranded (ds) DNA and anti-Sm/RNP. GST-P1 did not induce an anti-dsDNA response in these mice. These results demonstrate that RNAPI(S1)-NT, RNAPI(S1)-BD and La can participate in an anti-autoantigen/anti-DNA antibody loop during an SLE-like autoimmune response.

KW - Anti-70.kDa U1-RNP antibodies

KW - Anti-dsDNA antibodies

KW - Lupus

KW - RNA polymerase I

KW - SLE

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Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies

Abstract

Keywords

ASJC Scopus subject areas

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