Imaging system design for surgical guidance with near-infrared autofluorescence

Melanie McWade, Isaac J. Pence, Constantine Paras, Anita Mahadevan-Jansen

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We present a study to evaluate and compare three near-infrared (NIR) fluorescence imaging systems designed to provide intraoperative guidance around anatomical structures. The three systems adapted specifically for the application of endogenous NIR fluorescence detection were (1) a photomultiplier tube based NIR viewer, (2) a thermoelectrically cooled electron-multiplying charge-coupled device (CCD) camera, and (3) a clinical endoscope CCD camera system. Each system was evaluated on the basis of ease-of-use, cost, and system performance. The cooled CCD camera showed the highest contrast ratio, but is limited in utility by its bulk interface and high cost. The clinical endoscope camera showed flexibility in its field-of-view and provides the benefit of being sterilized to allow easy integration into the surgical suite; however, it exhibits signal nonlinearity that would distort quantitative analysis. The NIR viewer shows optimal performance, exhibiting high spatial resolution, linearity, and sufficient contrast to differentiate between tissue types. This low-cost design proves to be the optimal system for parathyroid detection, offering ease-of-use in a surgical setting while meeting system performance requirements.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII
EditorsTuan Vo-Dinh, Anita Mahadevan-Jansen, Warren S. Grundfest
ISBN (Electronic)9781628414035
StatePublished - 2015
Externally publishedYes
EventAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII - San Francisco, United States
Duration: Feb 8 2015Feb 10 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII
Country/TerritoryUnited States
CitySan Francisco


  • anatomical imaging
  • intraoperative imaging
  • near-infrared fluorescence
  • parathyroid detection
  • surgical guidance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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